| Literature DB >> 8981418 |
P Ballerini1, M P Rathbone, P Di Iorio, A Renzetti, P Giuliani, I D'Alimonte, O Trubiani, F Caciagli, R Ciccarelli.
Abstract
Treatment of rat astrocyte cultures with 2'- and 3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP), a P2X7 agonist, but not with adenosine 5-[alpha, beta methylene] triphosphate (alpha, beta meATP), a P2X agonist, increased influx of extracellular Ca2+ and [Ca2+]i. Lucifer yellow, a small molecule which permeates P2X7 receptor-induced pores, entered BzATP-treated but not control astrocytes. BzATP also stimulated efflux of [3H]purine from cultured astrocytes. The P2X7 receptor antagonist oxidized ATP abolished the effects of BzATP on [Ca2+]i, lucifer yellow permeation and [3H]purine release, indicating that these effects were due to P2X7 receptor activation. In neurological diseases or injuries extracellular ATP may activate P2X7 receptors further enhancing [3H]purine release, with important pathophysiological consequences.Entities:
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Year: 1996 PMID： 8981418 DOI： 10.1097/00001756-199611040-00026
Source DB: PubMed Journal: Neuroreport ISSN： 0959-4965 Impact factor: 1.837