| Literature DB >> 8980237 |
J Z Tsien1, D F Chen, D Gerber, C Tom, E H Mercer, D J Anderson, M Mayford, E R Kandel, S Tonegawa.
Abstract
Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.Entities:
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Year: 1996 PMID： 8980237 DOI： 10.1016/s0092-8674(00)81826-7
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582