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Literature DB >> 8979362

Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR.

Abstract

A species-specific gene probe for Hyphomicrobium facilis was generated from a transposon Tn5-132 insertion mutant defective in methanol oxidation by the inverse PCR. With this probe, the abundance of H. facilis in a garden soil was determined as a subfraction of the total cultivable hyphomicrobia.

Entities: Chemical Species

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Year: 1997 PMID： 8979362 PMCID： PMC168326 DOI： 10.1128/aem.63.1.335-337.1997

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

8 in total

1. BIOLOGY OF BUDDING BACTERIA. I. ENRICHMENT, ISOLATION AND MORPHOLOGY OF HYPHOMICROBIUM SPP.

Authors: P HIRSCH; S F CONTI
Journal: Arch Mikrobiol Date: 1964-06-26

2. A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria).

Authors: K F Jarrell; D Faguy; A M Hebert; M L Kalmokoff
Journal: Can J Microbiol Date: 1992-01 Impact factor: 2.419

3. Detection of methanotrophic bacteria in environmental samples with the PCR.

Authors: I R McDonald; E M Kenna; J C Murrell
Journal: Appl Environ Microbiol Date: 1995-01 Impact factor: 4.792

4. A single oligonucleotide can be used to rapidly isolate DNA sequences flanking a transposon Tn5 insertion by the polymerase chain reaction.

Authors: J J Rich; D K Willis
Journal: Nucleic Acids Res Date: 1990-11-25 Impact factor: 16.971

1 in total

1. The effect of nitrogen enrichment on c(1)-cycling microorganisms and methane flux in salt marsh sediments.

Authors: Irina C Irvine; Lucía Vivanco; Peris N Bentley; Jennifer B H Martiny
Journal: Front Microbiol Date: 2012-03-19 Impact factor: 5.640

1 in total

Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR.

1. BIOLOGY OF BUDDING BACTERIA. I. ENRICHMENT, ISOLATION AND MORPHOLOGY OF HYPHOMICROBIUM SPP.

2. A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria).

3. Detection of methanotrophic bacteria in environmental samples with the PCR.

4. A single oligonucleotide can be used to rapidly isolate DNA sequences flanking a transposon Tn5 insertion by the polymerase chain reaction.

5. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

6. Diversity and structure of hyphomicrobium populations in a sewage treatment plant and its adjacent receiving lake.

7. Mutagenesis and chromosome mobilization in Hyphomicrobium facilis B-522.

8. Vectors for transposon mutagenesis of non-enteric bacteria.

1. The effect of nitrogen enrichment on c(1)-cycling microorganisms and methane flux in salt marsh sediments.