I N Farstad1, J Norstein, P Brandtzaeg. 1. Laboratory for Immunohistochemistry and Immunopathology, University of Oslo, National Hospital, Norway.
Abstract
BACKGROUND & AIMS: Cells in lymph draining the human gut have not been characterized previously. The aim of this study was to phenotype B and T cells present in microlymphatics of Peyer's pathces and in mesenteric lymph. METHODS: The studies were conducted by multicolor immunohistochemistry, flow cytometry, and immunocytochemistry. RESULTS: In decreasing order of frequency, microlymphatics in Peyer's patches contained naive T (CD3+CD45RA+ alpha 4 beta 7low) and B (sIgD+CD20+ alpha 4 beta 7low) lymphocytes, memory T (CD45RO+ alpha 4 beta 7+) and B (sIgD-CD20+ alpha 4 beta 7+) lymphocytes, and B-cell blasts (CD19+CD38high alpha 4 beta 7high). Naive cells were usually positive for L-selectin, memory cells were either positive or negative, and B-cell blasts were usually negative. Mesenteric lymph contained naive T (approximately 60%) and B (approximately 25%) lymphocytes, memory T and B lymphocytes (approximately 10%), and B-cell blasts (approximately 2%). Cytospins confirmed these results and showed, in addition, that B-cell blasts contained cytoplasmic immunoglobulin (Ig) A, IgM, or IgG in overall proportions of 5:1: < 0.5. CONCLUSIONS: Our results are similar to the phenotypes previously described in animal thoracic or mesenteric lymph. A fraction of the B cells stimulated in Peyer's patches are near terminal differentiation (contain cytoplasmic Ig) before they enter peripheral blood. Many memory cells, and most if not all B-cell blasts entering lymph show an adhesion molecule profile (alpha 4 beta 7high L-selectin low) in keeping with the presumed phenotype of lymphoid cells destined for mucosal effector sites such as the gut lamina propria.
BACKGROUND & AIMS: Cells in lymph draining the human gut have not been characterized previously. The aim of this study was to phenotype B and T cells present in microlymphatics of Peyer's pathces and in mesenteric lymph. METHODS: The studies were conducted by multicolor immunohistochemistry, flow cytometry, and immunocytochemistry. RESULTS: In decreasing order of frequency, microlymphatics in Peyer's patches contained naive T (CD3+CD45RA+ alpha 4 beta 7low) and B (sIgD+CD20+ alpha 4 beta 7low) lymphocytes, memory T (CD45RO+ alpha 4 beta 7+) and B (sIgD-CD20+ alpha 4 beta 7+) lymphocytes, and B-cell blasts (CD19+CD38high alpha 4 beta 7high). Naive cells were usually positive for L-selectin, memory cells were either positive or negative, and B-cell blasts were usually negative. Mesenteric lymph contained naive T (approximately 60%) and B (approximately 25%) lymphocytes, memory T and B lymphocytes (approximately 10%), and B-cell blasts (approximately 2%). Cytospins confirmed these results and showed, in addition, that B-cell blasts contained cytoplasmic immunoglobulin (Ig) A, IgM, or IgG in overall proportions of 5:1: < 0.5. CONCLUSIONS: Our results are similar to the phenotypes previously described in animal thoracic or mesenteric lymph. A fraction of the B cells stimulated in Peyer's patches are near terminal differentiation (contain cytoplasmic Ig) before they enter peripheral blood. Many memory cells, and most if not all B-cell blasts entering lymph show an adhesion molecule profile (alpha 4 beta 7high L-selectin low) in keeping with the presumed phenotype of lymphoid cells destined for mucosal effector sites such as the gut lamina propria.
Authors: Urs M Mörbe; Peter B Jørgensen; Thomas M Fenton; Nicole von Burg; Lene B Riis; Jo Spencer; William W Agace Journal: Mucosal Immunol Date: 2021-03-22 Impact factor: 7.313
Authors: N Nair; E W Newell; C Vollmers; S R Quake; J M Morton; M M Davis; X S He; H B Greenberg Journal: Mucosal Immunol Date: 2015-04-22 Impact factor: 7.313