Literature DB >> 8975604

Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus.

D Cue1, H Lam, R S Hanson, M C Flickinger.   

Abstract

We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.

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Year:  1996        PMID： 8975604      PMCID： PMC167875          DOI： 10.1128/aem.62.3.1107-1111.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

Review 1.  Methylotrophs: genetics and commercial applications.

Authors:  M E Lidstrom; D I Stirling
Journal:  Annu Rev Microbiol       Date:  1990       Impact factor: 15.500

2.  A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.

Authors:  M Itaya; K Kondo; T Tanaka
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

3.  L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp.

Authors:  F J Schendel; C E Bremmon; M C Flickinger; M Guettler; R S Hanson
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

4.  Cloning and sequence analysis of the meso-diaminopimelate decarboxylase gene from Bacillus methanolicus MGA3 and comparison to other decarboxylase genes.

Authors:  D A Mills; M C Flickinger
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

5.  Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

Authors:  M Nelson; E Raschke; M McClelland
Journal:  Nucleic Acids Res       Date:  1993-07-01       Impact factor: 16.971

6.  Assaying of organisms for the presence of restriction endonucleases.

Authors:  R Schleif
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

8.  Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria.

Authors:  N Arfman; L Dijkhuizen; G Kirchhof; W Ludwig; K H Schleifer; E S Bulygina; K M Chumakov; N I Govorukhina; Y A Trotsenko; D White
Journal:  Int J Syst Bacteriol       Date:  1992-07

9.  Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp.

Authors:  F J Schendel; M C Flickinger
Journal:  Appl Environ Microbiol       Date:  1992-09       Impact factor: 4.792
  9 in total
  1 in total

1.  Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph.

Authors:  D Cue; H Lam; R L Dillingham; R S Hanson; M C Flickinger
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792
  1 in total

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