Literature DB >> 8970173

Primed in situ labeling (PRINS). A fast method for in situ labeling of nucleic acids.

J Hindkjaer1, J Koch, C Brandt, S Kølvraa, L Bolund.   

Abstract

PRimed IN Situ labeling (PRINS) is a fast and sensitive alternative to fluorescence in situ hybridization (FISH) for identification of chromosome aberrations. In this article, we present the detailed protocols for detection of repeat sequences using oligonucleotides or fragments of cloned probes as primers for PRINS. We describe a multicolor PRINS procedure for simultaneous visualization of more probes in different colors on a metaphase preparation, and a PRINS-painting procedure, which combines PRINS and chromosome painting. Finally, a protocol for detection of single-copy genes is presented.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8970173     DOI： 10.1007/BF02740774

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  16 in total

Review 1.  A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence.

Authors:  K H Choo; B Vissel; A Nagy; E Earle; P Kalitsis
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

2.  An improved method for chromosome-specific labeling of alpha satellite DNA in situ by using denatured double-stranded DNA probes as primers in a primed in situ labeling (PRINS) procedure.

Authors:  J Koch; J Hindkjaer; J Mogensen; S Kølvraa; L Bolund
Journal:  Genet Anal Tech Appl       Date:  1991-09

3.  A signal of telomeric sequences on porcine chromosome 6q21-q22 detected by primed in situ labelling.

Authors:  F Gu; J Hindkjaer; I Gustavsson; L Bolund
Journal:  Chromosome Res       Date:  1996-04       Impact factor: 5.239

4.  Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome.

Authors:  J S Waye; H F Willard
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

5.  Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Authors:  D Pinkel; J Landegent; C Collins; J Fuscoe; R Segraves; J Lucas; J Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

6.  Simultaneous detection of centromere-specific probes and chromosome painting libraries by a combination of primed in situ labelling and chromosome painting (PRINS-painting).

Authors:  J Hindkjaer; C A Brandt; J Koch; T B Lund; S Kølvraa; L Bolund
Journal:  Chromosome Res       Date:  1995-01       Impact factor: 5.239

7.  Fast, sensitive multicolor detection of nucleic acids in situ by PRimed IN Situ labeling (PRINS).

Authors:  J Hindkjaer; J Koch; C Terkelsen; C A Brandt; S Kølvraa; L Bolund
Journal:  Cytogenet Cell Genet       Date:  1994

8.  Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ.

Authors:  J E Koch; S Kølvraa; K B Petersen; N Gregersen; L Bolund
Journal:  Chromosoma       Date:  1989-10       Impact factor: 4.316

9.  Construction of a panel of chromosome-specific oligonucleotide probes (PRINS-primers) useful for the identification of individual human chromosomes in situ.

Authors:  J Koch; J Hindkjaer; S Kølvraa; L Bolund
Journal:  Cytogenet Cell Genet       Date:  1995

10.  Variable (dG-dT)n.(dC-dA)n sequences in the porcine genome.

Authors:  A K Winterø; M Fredholm; P D Thomsen
Journal:  Genomics       Date:  1992-02       Impact factor: 5.736
View more
  1 in total

1.  Visualization of oligonucleotide probes and point mutations in interphase nuclei and DNA fibers using rolling circle DNA amplification.

Authors:  X B Zhong ; P M Lizardi; X H Huang ; P L Bray-Ward; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  2001-03-27       Impact factor: 11.205
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.