BACKGROUND & AIMS: Helicobacter pylori strains possessing the cagA gene are thought to be associated with gastroduodenal diseases. Furthermore, some cytokines are considered to play a role in gastric mucosal inflammation. The aim of this study was to investigate the relationship between cagA gene and cytokine messenger RNA (mRNA) expression in gastric mucosa. METHODS: In 160 patients, the cagA gene was detected using polymerase chain reaction, and interleukin (IL) 1 beta, IL-6, IL-7, IL-8, IL-10, and tumor necrosis factor (TNF) alpha mRNA were detected using reverse-transcription polymerase chain reaction. RESULTS: Specimens infected with cagA gene-positive strains (cagA-positive specimens) had significantly more severe infiltration of polymorphonuclear leukocytes and mononuclear cells than those infected with cagA gene-negative strains (cagA-negative specimens). Levels of expression of IL-6, IL-7, IL-8, IL-10, and TNF-alpha mRNA were significantly higher in H. pylori-positive than H. pylori-negative patients. Furthermore, the level of IL-8 mRNA expression was significantly higher in cagA-positive than cagA-negative specimens. CONCLUSIONS: cagA-positive strains induce the expression of IL-8 mRNA, suggesting that IL-8 may play important roles in the pathogenesis of gastroduodenal diseases associated with H. pylori infection.
BACKGROUND & AIMS:Helicobacter pylori strains possessing the cagA gene are thought to be associated with gastroduodenal diseases. Furthermore, some cytokines are considered to play a role in gastric mucosal inflammation. The aim of this study was to investigate the relationship between cagA gene and cytokine messenger RNA (mRNA) expression in gastric mucosa. METHODS: In 160 patients, the cagA gene was detected using polymerase chain reaction, and interleukin (IL) 1 beta, IL-6, IL-7, IL-8, IL-10, and tumor necrosis factor (TNF) alpha mRNA were detected using reverse-transcription polymerase chain reaction. RESULTS: Specimens infected with cagA gene-positive strains (cagA-positive specimens) had significantly more severe infiltration of polymorphonuclear leukocytes and mononuclear cells than those infected with cagA gene-negative strains (cagA-negative specimens). Levels of expression of IL-6, IL-7, IL-8, IL-10, and TNF-alpha mRNA were significantly higher in H. pylori-positive than H. pylori-negative patients. Furthermore, the level of IL-8 mRNA expression was significantly higher in cagA-positive than cagA-negative specimens. CONCLUSIONS:cagA-positive strains induce the expression of IL-8 mRNA, suggesting that IL-8 may play important roles in the pathogenesis of gastroduodenal diseases associated with H. pyloriinfection.
Authors: M Eck; B Schmausser; K Scheller; A Toksoy; M Kraus; T Menzel; H K Müller-Hermelink; R Gillitzer Journal: Clin Exp Immunol Date: 2000-11 Impact factor: 4.330
Authors: T Tanahashi; M Kita; T Kodama; Y Yamaoka; N Sawai; T Ohno; S Mitsufuji; Y P Wei; K Kashima; J Imanishi Journal: Infect Immun Date: 2000-02 Impact factor: 3.441
Authors: Anna Lundgren; Christina Trollmo; Anders Edebo; Ann-Mari Svennerholm; B Samuel Lundin Journal: Infect Immun Date: 2005-09 Impact factor: 3.441