Molded continuous poly(styrene-co-divinylbenzene) rod as a separation medium for the very fast separation of polymers. Comparison of the chromatographic properties of the monolithic rod with columns packed with porous and non-porous beads in high-performance liquid chromatography of polystyrenes.

Gradient elution separations of polystyrene standards in a monolithic molded 50 x 8 mm I.D. poly(styrene-co-divinylbenzene) rod column and in 50 x 8 mm I.D. and 30 x 4.1 mm I.D. columns packed with porous and non-porous poly(styrene-co-divinylbenzene) beads has been carried out. All of these separation media differ in shape and porosity. Excellent separations of eight polystyrene standards were achieved with both the molded monolithic rod and porous beads at moderate flow-rates. However, the monolithic medium proved to be superior for high-speed separations using very steep gradients at a flow-rate of 20 ml/min. Three polystyrene standards were separated in the rod column within 4 s. The separation in the column packed with non-porous beads was poor at flow-rates of 2-8 ml/min, while higher flow-rates led to an unacceptably high back pressure.