Literature DB >> 8961565

A method for the large scale isolation of high transformation efficiency fungal genomic DNA.

D Zhang1, Y Yang, L A Castlebury, C E Cerniglia.   

Abstract

A procedure for isolation of genomic DNA from the zygomycete Cunninghamella elegans and other filamentous fungi and yeasts is reported. This procedure involves disruption of cells by grinding using dry ice, removal of polysaccharides using cetyltrimethylammonium bromide and by phenol extractions, and precipitation of DNA with isopropanol at room temperature. The isolation method produced large scale (approximate 1 mg DNA/5 g wet cells) and highly purified high molecular mass DNA. Sau3AI partially digested DNA showed high transformation efficiency (> 10(6)/100 ng DNA) when ligated to ZAP-express lambda vector.

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Year:  1996        PMID： 8961565     DOI： 10.1111/j.1574-6968.1996.tb08587.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  19 in total

1.  Identification of Pythium insidiosum by nested PCR in cutaneous lesions of Brazilian horses and rabbits.

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Journal:  Curr Microbiol       Date:  2010-12-25       Impact factor: 2.188

2.  The T788G mutation in the cyp51C gene confers voriconazole resistance in Aspergillus flavus causing aspergillosis.

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Journal:  Antimicrob Agents Chemother       Date:  2012-02-06       Impact factor: 5.191

3.  Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis.

Authors:  Kaihui Liu; Xiaowei Ding; Baiwan Deng; Wenqiang Chen
Journal:  J Ind Microbiol Biotechnol       Date:  2009-05-30       Impact factor: 3.346

4.  Fungal endophyte diversity and bioactivity in the Mediterranean cypress Cupressus sempervirens.

Authors:  Jalal Soltani; Mahdieh S Hosseyni Moghaddam
Journal:  Curr Microbiol       Date:  2014-12-20       Impact factor: 2.188

5.  Isolation and characterization of endophytic huperzine A-producing fungi from Huperzia serrata.

Authors:  Ya Wang; Qing Gui Zeng; Zhi Bin Zhang; Ri Ming Yan; Ling Yun Wang; Du Zhu
Journal:  J Ind Microbiol Biotechnol       Date:  2010-11-24       Impact factor: 3.346

6.  Isolation and characterization of formaldehyde-degrading fungi and its formaldehyde metabolism.

Authors:  Diansi Yu; Lili Song; Wei Wang; Changhong Guo
Journal:  Environ Sci Pollut Res Int       Date:  2014-01-25       Impact factor: 4.223

7.  Isolation, diversity and acetylcholinesterase inhibitory activity of the culturable endophytic fungi harboured in Huperzia serrata from Jinggang Mountain, China.

Authors:  Ya Wang; Zheng Lai; Xi-Xi Li; Ri-Ming Yan; Zhi-Bin Zhang; Hui-Lin Yang; Du Zhu
Journal:  World J Microbiol Biotechnol       Date:  2016-01-08       Impact factor: 3.312

8.  Identification of a novel endophytic fungus from Huperzia serrata which produces huperzine A.

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Journal:  World J Microbiol Biotechnol       Date:  2014-09-12       Impact factor: 3.312

9.  Diversity and Spatiotemporal Distribution of Fungal Endophytes Associated with Citrus reticulata cv. Siyahoo.

Authors:  Fatemeh Sadeghi; Davood Samsampour; Majeed Askari Seyahooei; Abdoolnabi Bagheri; Jalal Soltani
Journal:  Curr Microbiol       Date:  2019-01-28       Impact factor: 2.188

10.  High infectivity of an endoparasitic fungus strain, Esteya vermicola, against nematodes.

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Journal:  J Microbiol       Date:  2008-08-31       Impact factor: 3.422
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