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Literature DB >> 8961506

Physicochemical and immunological studies on the stability of free and microsphere-encapsulated tetanus toxoid in vitro.

D K Xing¹, D T Crane, B Bolgiano, M J Corbel, C Jones, D Sesardic.

Abstract

The stability of tetanus toxoid released from experimental, slow-release microsphere vaccines was compared with that of free toxoid under different conditions over a 3 month period. The amount of antigenicity remaining, as measured by ELISA, correlated well with loss of toxoid structure, as determined by circular dichroism and fluorescence spectroscopy. At 37 degrees C and low pH, pH 2.5 for free toxoid, or under the acidic conditions generated by the hydrolysis of fast-release microspheres, a gradual unfolding of the polypeptide chain was observed within the first few weeks with more rapid denaturation beyond 30 days.

Entities: Disease

Mesh：

Substances：
Tetanus Toxoid

Year: 1996 PMID： 8961506 DOI： 10.1016/s0264-410x(96)00032-1

Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641

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Cited

11 in total

1. pH and osmotic pressure inside biodegradable microspheres during erosion.

Authors: A Brunner; K Mäder; A Göpferich
Journal: Pharm Res Date: 1999-06 Impact factor: 4.200

Review 2. Protein instability in poly(lactic-co-glycolic acid) microparticles.

Authors: M van de Weert; W E Hennink; W Jiskoot
Journal: Pharm Res Date: 2000-10 Impact factor: 4.200

3. Improving stability and release kinetics of microencapsulated tetanus toxoid by co-encapsulation of additives.

Authors: P Johansen; Y Men; R Audran; G Corradin; H P Merkle; B Gander
Journal: Pharm Res Date: 1998-07 Impact factor: 4.200

Review 4. Stability of protein pharmaceuticals: an update.

Authors: Mark Cornell Manning; Danny K Chou; Brian M Murphy; Robert W Payne; Derrick S Katayama
Journal: Pharm Res Date: 2010-02-09 Impact factor: 4.200

Review 5. Technologies to improve immunisation safety.

Authors: C J Clements; M T Aguado; L Jódar
Journal: Drug Saf Date: 2001 Impact factor: 5.606

6. Tetanus toxoid-loaded layer-by-layer nanoassemblies for efficient systemic, mucosal, and cellular immunostimulatory response following oral administration.

Authors: Harshad Harde; Ashish Kumar Agrawal; Sanyog Jain
Journal: Drug Deliv Transl Res Date: 2015-10 Impact factor: 4.617

Physicochemical and immunological studies on the stability of free and microsphere-encapsulated tetanus toxoid in vitro.

1. pH and osmotic pressure inside biodegradable microspheres during erosion.

Review 2. Protein instability in poly(lactic-co-glycolic acid) microparticles.

3. Improving stability and release kinetics of microencapsulated tetanus toxoid by co-encapsulation of additives.

Review 4. Stability of protein pharmaceuticals: an update.

Review 5. Technologies to improve immunisation safety.

6. Tetanus toxoid-loaded layer-by-layer nanoassemblies for efficient systemic, mucosal, and cellular immunostimulatory response following oral administration.

7. Hollow Microparticles as a Superior Delivery System over Solid Microparticles for the Encapsulation of Peptides.

Review 8. Vaccine adjuvants: current challenges and future approaches.

9. Stabilization of tetanus toxoid encapsulated in PLGA microspheres.

10. Structural correlates of carrier protein recognition in tetanus toxoid-conjugated bacterial polysaccharide vaccines.