N A Ahmed1, N V Christou. 1. Department of Surgery, McGill University, Montreal, Que.
Abstract
OBJECTIVE: To determine whether the expression of L-selectin is reduced in patients with systemic inflammatory response syndrome (SIRS) compared with control subjects, to test the effect of exudation of neutrophil L-selectin expression, and to measure soluble serum concentrations of shed L-selectin in the two groups. DESIGN: Prospective study. SETTING: Intensive care unit in a tertiary care hospital (Royal Victoria Hospital, Montreal). PATIENTS: Twenty-five patients with SIRS and 20 healthy, age-matched controls. INTERVENTIONS: Collection of exudate neutrophils from skin window and circulating neutrophils from venous blood. OUTCOME MEASURES: Neutrophil L-selectin levels, measured with the use of fluorocytometry, and soluble L-selectin levels, measured with the use of enzymelinked immunosorbent assay. RESULTS: There is a significant reduction in L-selectin expression on circulating neutrophils in patients with SIRS, compared with control subjects. Exudation of neutrophils to an extravascular site resulted in a dramatic down-regulation of L-selectin in both groups. Serum levels of soluble L-selectin were higher in patients with SIRS than in control subjects. CONCLUSIONS: The loss of L-selectin may be partly responsible for reduced neutrophil exudation to extravascular sites in patients with SIRS.
OBJECTIVE: To determine whether the expression of L-selectin is reduced in patients with systemic inflammatory response syndrome (SIRS) compared with control subjects, to test the effect of exudation of neutrophil L-selectin expression, and to measure soluble serum concentrations of shed L-selectin in the two groups. DESIGN: Prospective study. SETTING: Intensive care unit in a tertiary care hospital (Royal Victoria Hospital, Montreal). PATIENTS: Twenty-five patients with SIRS and 20 healthy, age-matched controls. INTERVENTIONS: Collection of exudate neutrophils from skin window and circulating neutrophils from venous blood. OUTCOME MEASURES: Neutrophil L-selectin levels, measured with the use of fluorocytometry, and soluble L-selectin levels, measured with the use of enzymelinked immunosorbent assay. RESULTS: There is a significant reduction in L-selectin expression on circulating neutrophils in patients with SIRS, compared with control subjects. Exudation of neutrophils to an extravascular site resulted in a dramatic down-regulation of L-selectin in both groups. Serum levels of soluble L-selectin were higher in patients with SIRS than in control subjects. CONCLUSIONS: The loss of L-selectin may be partly responsible for reduced neutrophil exudation to extravascular sites in patients with SIRS.
Authors: G Vitale; S Mansueto; G Gambino; C Mocciaro; C La Russa; P Mansueto; M A Zambito; V Ferlazzo; C Barbera; M La Rosa; S Milano; E Cillari Journal: Clin Exp Immunol Date: 1999-08 Impact factor: 4.330