Literature DB >> 8957552

Enhanced polymer one-step staining for proliferating cell nuclear antigen in squamous cell carcinoma of the tongue.

K Sano1, J Sekine, T Inokuchi, M B Pe, G Ma.   

Abstract

A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopathology and in performing double immunostaining.

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Year:  1996        PMID： 8957552     DOI： 10.3109/10520299609117174

Source DB:  PubMed          Journal:  Biotech Histochem        ISSN： 1052-0295            Impact factor:   1.718


  4 in total

1.  Selective cyclooxygenase-2 blocker delays healing of esophageal ulcers in rats and inhibits ulceration-triggered c-Met/hepatocyte growth factor receptor induction and extracellular signal-regulated kinase 2 activation.

Authors:  Dolgor Baatar; Michael K Jones; Rama Pai; Hirofumi Kawanaka; Imre L Szabo; Woo S Moon; Seigo Kitano; Andrzej S Tarnawski
Journal:  Am J Pathol       Date:  2002-03       Impact factor: 4.307

2.  Novel mechanisms and signaling pathways of esophageal ulcer healing: the role of prostaglandin EP2 receptors, cAMP, and pCREB.

Authors:  Amrita Ahluwalia; Dolgor Baatar; Michael K Jones; Andrzej S Tarnawski
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2014-07-24       Impact factor: 4.052

3.  Esophageal ulceration triggers expression of hypoxia-inducible factor-1 alpha and activates vascular endothelial growth factor gene: implications for angiogenesis and ulcer healing.

Authors:  Dolgor Baatar; Michael K Jones; Koji Tsugawa; Rama Pai; Woo S Moon; Gou Y Koh; Injune Kim; Seigo Kitano; Andrzej S Tarnawski
Journal:  Am J Pathol       Date:  2002-10       Impact factor: 4.307

4.  Antibody validation of immunohistochemistry for biomarker discovery: recommendations of a consortium of academic and pharmaceutical based histopathology researchers.

Authors:  William J Howat; Arthur Lewis; Phillipa Jones; Caroline Kampf; Fredrik Pontén; Chris M van der Loos; Neil Gray; Chris Womack; Anthony Warford
Journal:  Methods       Date:  2014-02-11       Impact factor: 3.608
  4 in total

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