Genomic organization and chromosomal location of murine Cdc42.

cdc42 is a member of the rho family of small GTPases, which are implicated as regulators of cellular morphology. To date, one murine and two human cdc42 isoforms have been identified. Here we report the cloning of a second murine isoform and provide evidence that the two isoforms arise from a single gene by alternative splicing. In contrast with the previously identified murine cdc42 sequence, which is expressed in a wide variety of tissues, the second isoform appears to be expressed exclusively in brain. Using single-strand conformation polymorphism analysis of a mouse backcross panel, the gene encoding cdc42 has been localized to distal chromosome 4.