Literature DB >> 8953222

A 3D NOESY-(HCACO)NH experiment for the measurement of NOEs involving 1H alpha in 13C/15N-labeled proteins dissolved in H2O.

W Zhang1, W H Gmeiner.   

Abstract

A 3D NOESY-(HCACO)NH experiment is described that transfers NOEs from 1H alpha to the backbone 1Hn in the succeeding residue for detection. Using this strategy, NOEs involving 1H alpha protons that resonate exactly at the water frequency can be detected. NOEs from an overlapping 1H alpha proton that is attached to degenerate 13C alpha can also be resolved. The performance of this approach is demonstrated for the 13C-/15N-labeled Hck/SH2 dissolved in H2O.

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Year:  1996        PMID： 8953222     DOI： 10.1007/bf00410334

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  4 in total

1.  Four-dimensional 13C/13C-edited nuclear Overhauser enhancement spectroscopy of a protein in solution: application to interleukin 1 beta.

Authors:  G M Clore; L E Kay; A Bax; A M Gronenborn
Journal:  Biochemistry       Date:  1991-01-08       Impact factor: 3.162

2.  Separation of NOEs from degenerate amide protons in 13C/15N-labeled proteins using a 3D 13C'-edited NOESY-H(N)CO experiment.

Authors:  W Zhang; T E Smithgall; W H Gmeiner
Journal:  J Magn Reson B       Date:  1996-06

3.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

Authors:  M Piotto; V Saudek; V Sklenár
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835

4.  Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution.

Authors:  L E Kay; G M Clore; A Bax; A M Gronenborn
Journal:  Science       Date:  1990-07-27       Impact factor: 47.728
  4 in total
  1 in total

1.  Three-dimensional structure of the Hck SH2 domain in solution.

Authors:  W Zhang; T E Smithgall; W H Gmeiner
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835
  1 in total

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