Literature DB >> 8953162

High-pH anion-exchange chromatography with pulsed amperometric detection and molar response factors of human milk oligosaccharides.

C Kunz1, S Rudloff, A Hintelmann, G Pohlentz, H Egge.   

Abstract

A method is described to separate and characterize neutral and acidic lactose-derived oligosaccharides without prior derivatization or reduction by high-pH anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). This method has been applied to human milk oligosaccharides from donors with different blood group specificity (A, Le(a) and A, Le(b). Neutral and acidic components were separated from each other by anion-exchange chromatography. A distinct separation of individual components was obtained by size-exclusion chromatography on Fractogel TSK HW 50S (acidic oligosaccharides) or Fractogel TSK HW 40S (neutral oligosaccharides containing up to 6 monomers) and Bio-Gel P-4 size exclusion (neutral oligosaccharides containing more than 6 monomers). Furthermore the moral response factors after HPAEC-PAD have been determined for 28 components.

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Year:  1996        PMID： 8953162     DOI： 10.1016/s0378-4347(96)00181-8

Source DB:  PubMed          Journal:  J Chromatogr B Biomed Appl        ISSN： 1572-6495


  19 in total

1.  Avidity of α-fucose on human milk oligosaccharides and blood group-unrelated oligo/polyfucoses is essential for potent norovirus-binding targets.

Authors:  Franz-Georg Hanisch; Grant S Hansman; Vasily Morozov; Clemens Kunz; Horst Schroten
Journal:  J Biol Chem       Date:  2018-06-01       Impact factor: 5.157

Review 2.  Human milk oligosaccharides: every baby needs a sugar mama.

Authors:  Lars Bode
Journal:  Glycobiology       Date:  2012-04-18       Impact factor: 4.313

3.  Simultaneous quantification of sialyloligosaccharides from human milk by capillary electrophoresis.

Authors:  Yuanwu Bao; Libin Zhu; David S Newburg
Journal:  Anal Biochem       Date:  2007-07-07       Impact factor: 3.365

4.  Enzymatic synthesis of fucose-containing galacto-oligosaccharides using β-galactosidase and identification of novel disaccharide structures.

Authors:  Anne Usvalampi; Hannu Maaheimo; Olli Tossavainen; Alexander D Frey
Journal:  Glycoconj J       Date:  2017-09-13       Impact factor: 2.916

5.  Quantification of neutral human milk oligosaccharides by graphitic carbon high-performance liquid chromatography with tandem mass spectrometry.

Authors:  Yuanwu Bao; Ceng Chen; David S Newburg
Journal:  Anal Biochem       Date:  2012-10-12       Impact factor: 3.365

6.  Short communication: Quantification of carbohydrates in whey permeate products using high-performance anion-exchange chromatography with pulsed amperometric detection.

Authors:  Hyeyoung Lee; Vitor Luiz de MeloSilva; Yan Liu; Daniela Barile
Journal:  J Dairy Sci       Date:  2015-09-09       Impact factor: 4.034

7.  The role of milk sialyllactose in intestinal bacterial colonization.

Authors:  G Adrienne Weiss; Thierry Hennet
Journal:  Adv Nutr       Date:  2012-05-01       Impact factor: 8.701

Review 8.  Historical aspects of human milk oligosaccharides.

Authors:  Clemens Kunz
Journal:  Adv Nutr       Date:  2012-05-01       Impact factor: 8.701

Review 9.  Human milk oligosaccharides and Lewis blood group: individual high-throughput sample profiling to enhance conclusions from functional studies.

Authors:  Dennis Blank; Viktoria Dotz; Rudolf Geyer; Clemens Kunz
Journal:  Adv Nutr       Date:  2012-05-01       Impact factor: 8.701

10.  Sialic acid utilisation and synthesis in the neonatal rat revisited.

Authors:  Peter I Duncan; Frédéric Raymond; Andreas Fuerholz; Norbert Sprenger
Journal:  PLoS One       Date:  2009-12-11       Impact factor: 3.240
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