Literature DB >> 8943051

In vitro V(D)J recombination: signal joint formation.

P Cortes1, F Weis-Garcia, Z Misulovin, A Nussenzweig, J S Lai, G Li, M C Nussenzweig, D Baltimore.   

Abstract

The first step of V(D)J recombination, specific cleavage at the recombination signal sequence (RSS), can be carried out by the recombination activating proteins RAG1 and RAG2. In vivo, the cleaved coding and signal ends must be rejoined to generate functional antigen receptors and maintain chromosomal integrity. We have investigated signal joint formation using deletion and inversion substrates in a cell free system. RAG1 and RAG2 alone or in combination were unable to generate signal joints. However, RAG1 and RAG2 complemented with nuclear extracts were able to recombine an extrachromosomal substrate and form precise signal joints. The in vitro reaction resembled authentic V(D)J recombination in being Ku-antigen-dependent.

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Year:  1996        PMID： 8943051      PMCID： PMC19485          DOI： 10.1073/pnas.93.24.14008

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  30 in total

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