Literature DB >> 894107

A simple rapid radiometric assay for pyrimidine-5'-nucleotidase.

J Torrance, C West, E Beutler.   

Abstract

Pyrimidine-5'-nucleotidase deficiency appears to be an important cause of hemolytic anemia associated with basophilic stippling of the red cells. A new radiometric method for the assay of this enzyme has been developed. In this technique, 14C-CMP serves as substrate. The CMP which is not dephosphorylated to cytidine is bound to the barium sulfate precipitate which forms in the deproteinization process. The cytidine remains in solution and is counted. The method is simple and reproducible and can be carried out on large numbers of samples. Two patients with pyrimidine-5'-nucleotidase deficiency have been detected by means of this technique.

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Year:  1977        PMID： 894107

Source DB:  PubMed          Journal:  J Lab Clin Med        ISSN： 0022-2143


  9 in total

1.  Determination of pyrimidine 5'-nucleotidase (P5N) activity in whole blood as an index of lead exposure.

Authors:  T Sakai; T Araki; K Ushio
Journal:  Br J Ind Med       Date:  1988-06

2.  Determination of erythrocyte pyrimidine 5'-nucleotidase activity by 31P nuclear magnetic resonance: comparison of normal controls and multiple sclerosis patients.

Authors:  G L Mendz; C R Middlehurst; P W Kuchel; P A Crossie
Journal:  Experientia       Date:  1986-09-15

3.  Adenine nucleotide degradation by the obligate intracellular bacterium Rickettsia typhi.

Authors:  J C Williams
Journal:  Infect Immun       Date:  1980-04       Impact factor: 3.441

4.  Lead poisoning. Further observations on erythrocyte pyrimidine-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotides.

Authors:  D E Paglia; W N Valentine; K Fink
Journal:  J Clin Invest       Date:  1977-12       Impact factor: 14.808

5.  A simplified method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N) activity by HPLC and its value in monitoring lead exposure.

Authors:  T Sakai; K Ushio
Journal:  Br J Ind Med       Date:  1986-12

6.  Detection of pyrimidine 5'-nucleotidase deficiency using 1H- or 31P-nuclear magnetic resonance.

Authors:  T Kagimoto; K Shirono; T Higaki; T Oda; H Matsuzaki; K Nagata; T Nakaji; Y Morino; K Takatsuki
Journal:  Experientia       Date:  1986-01-15

7.  Deficiency of pyrimidine 5'-nucleotidase in human leukocytes.

Authors:  D de Korte; C C van Doorn; J M Sijstermans; A H van Gennip; D Roos
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

8.  Evaluation of lead exposure in workers at a lead-acid battery factory in Korea: with focus on activity of erythrocyte pyrimidine 5'-nucleotidase (P5N).

Authors:  Y Kim; K Harada; S Ohmori; B K Lee; H Miura; A Ueda
Journal:  Occup Environ Med       Date:  1995-07       Impact factor: 4.402

9.  Studies on erythrocyte pyrimidine 5'-nucleotidase (P5N) test and its evaluation in workers occupationally exposed to lead.

Authors:  M Ichiba; K Tomokuni
Journal:  Int Arch Occup Environ Health       Date:  1990       Impact factor: 3.015
  9 in total

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