| Literature DB >> 8939627 |
M J Weickert1, D H Doherty, E A Best, P O Olins.
Abstract
Escherichia coli has long been the primary prokaryotic host for the synthesis of heterologous proteins. Recent advances have been made in the expression of complex proteins as soluble, functional molecules, complete with prosthetic groups, disulfide bonds, and quaternary structure. The development of alternative promoter and induction strategies has improved the options available for manipulating the expression conditions, which are frequently critical to soluble yield.Entities:
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Year: 1996 PMID： 8939627 DOI： 10.1016/s0958-1669(96)80051-6
Source DB: PubMed Journal: Curr Opin Biotechnol ISSN： 0958-1669 Impact factor: 9.740