Literature DB >> 8935654

Transformation of the extremely thermoacidophilic archaeon Sulfolobus solfataricus via a self-spreading vector.

M G Elferink1, C Schleper, W Zillig.   

Abstract

We describe a transformation system for extremely thermophilic archaea of the genus Sulfolobus in the kingdom Crenarchaeota. We have constructed in vitro a recombinant derivative of the recently described conjugative plasmid pNOB8, containing a beta-galactosidase gene downstream of a strong promotor. Transformation of a beta-galactosidase negative mutant of Sulfolobus solfataricus with this construct resulted in its spreading through the culture containing the primary transformants and in efficient restoration of beta-galactosidase activity.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8935654     DOI： 10.1111/j.1574-6968.1996.tb08078.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  9 in total

1.  High spontaneous mutation rate in the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by transposable elements.

Authors:  E Martusewitsch; C W Sensen; C Schleper
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

2.  Characterization and functional complementation of a nonlethal deletion in the chromosome of a beta-glycosidase mutant of Sulfolobus solfataricus.

Authors:  Simonetta Bartolucci; Mosè Rossi; Raffaele Cannio
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

3.  Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance.

Authors:  Rie Matsumi; Kenji Manabe; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal:  J Bacteriol       Date:  2007-01-26       Impact factor: 3.490

4.  Targeted gene disruption by homologous recombination in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

Authors:  Takaaki Sato; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

Review 5.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

6.  A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus.

Authors:  Tiziana Aucelli; Patrizia Contursi; Michele Girfoglio; Mosè Rossi; Raffaele Cannio
Journal:  Nucleic Acids Res       Date:  2006-09-13       Impact factor: 16.971

Review 7.  Sulfolobus - A Potential Key Organism in Future Biotechnology.

Authors:  Julian Quehenberger; Lu Shen; Sonja-Verena Albers; Bettina Siebers; Oliver Spadiut
Journal:  Front Microbiol       Date:  2017-12-12       Impact factor: 5.640

8.  The biology of thermoacidophilic archaea from the order Sulfolobales.

Authors:  April M Lewis; Alejandra Recalde; Christopher Bräsen; James A Counts; Phillip Nussbaum; Jan Bost; Larissa Schocke; Lu Shen; Daniel J Willard; Tessa E F Quax; Eveline Peeters; Bettina Siebers; Sonja-Verena Albers; Robert M Kelly
Journal:  FEMS Microbiol Rev       Date:  2021-08-17       Impact factor: 16.408

9.  Overview of the genetic tools in the Archaea.

Authors:  Haruyuki Atomi; Tadayuki Imanaka; Toshiaki Fukui
Journal:  Front Microbiol       Date:  2012-10-02       Impact factor: 5.640
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.