Literature DB >> 8931320

Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA.

V Ramisse1, G Patra, H Garrigue, J L Guesdon, M Mock.   

Abstract

Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isolates, and simultaneous confirmation of the species identity independent of plasmid content. The assay amplifies lef, cya, pag (pXO1) and cap (pXO2) genes, and a B. anthracis specific chromosomal marker, giving an easy-to-read profile. This system unambiguously identified virulent (pXO1+/2+) and avirulent (pXO1+/2-, pXO1-/2+ and pXO1-/2-) strains of B. anthracis and distinguished "anthrax-like' strains from other B. cereus group bacteria.

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Year:  1996        PMID： 8931320     DOI： 10.1111/j.1574-6968.1996.tb08548.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  54 in total

1.  Homoduplex and heteroduplex polymorphisms of the amplified ribosomal 16S-23S internal transcribed spacers describe genetic relationships in the "Bacillus cereus group".

Authors:  D Daffonchio; A Cherif; S Borin
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Antibiotic susceptibilities of 96 isolates of Bacillus anthracis isolated in France between 1994 and 2000.

Authors:  Jean-Didier Cavallo; Francoise Ramisse; Monique Girardet; Josée Vaissaire; Michelle Mock; Eric Hernandez
Journal:  Antimicrob Agents Chemother       Date:  2002-07       Impact factor: 5.191

3.  Bovine Bacillus anthracis in Cameroon.

Authors:  Paola Pilo; Alexandra Rossano; Hamadou Bamamga; Souley Abdoulkadiri; Vincent Perreten; Joachim Frey
Journal:  Appl Environ Microbiol       Date:  2011-06-24       Impact factor: 4.792

4.  Multiplex PCR for species-level identification of Bacillus anthracis and detection of pXO1, pXO2, and related plasmids.

Authors:  Marco A Riojas; Katalin Kiss; Marian L McKee; Manzour Hernando Hazbón
Journal:  Health Secur       Date:  2015-03-20

5.  Protocol for real-time PCR identification of anthrax spores from nasal swabs after broth enrichment.

Authors:  Marco R Oggioni; Francesca Meacci; Alessandra Carattoli; Alessandra Ciervo; Germano Orru; Antonio Cassone; Gianni Pozzi
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

6.  Non-additive costs and interactions alter the competitive dynamics of co-occurring ecologically distinct plasmids.

Authors:  Elise R Morton; Thomas G Platt; Clay Fuqua; James D Bever
Journal:  Proc Biol Sci       Date:  2014-02-05       Impact factor: 5.349

7.  Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis.

Authors:  Y Qi; G Patra; X Liang; L E Williams; S Rose; R J Redkar; V G DelVecchio
Journal:  Appl Environ Microbiol       Date:  2001-08       Impact factor: 4.792

8.  PCR assay to detect Bacillus anthracis spores in heat-treated specimens.

Authors:  A Fasanella; S Losito; R Adone; F Ciuchini; T Trotta; S A Altamura; D Chiocco; G Ippolito
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

9.  Detection of Bacillus anthracis DNA by LightCycler PCR.

Authors:  Constance A Bell; James R Uhl; Ted L Hadfield; John C David; Richard F Meyer; Thomas F Smith; Franklin R Cockerill
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

10.  Evaluation and validation of a real-time polymerase chain reaction assay for rapid identification of Bacillus anthracis.

Authors:  Alex R Hoffmaster; Richard F Meyer; Michael D Bowen; Chung K Marston; Robbin S Weyant; Kathy Thurman; Sharon L Messenger; Erin E Minor; Jonas M Winchell; Max V Rassmussen; Bruce R Newton; J Todd Parker; William E Morrill; Nancy McKinney; Gwen A Barnett; James J Sejvar; John A Jernigan; Bradley A Perkins; Tanja Popovic
Journal:  Emerg Infect Dis       Date:  2002-10       Impact factor: 6.883
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