| Literature DB >> 8926336 |
Abstract
Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scaleable to 600 1 volume.Mesh：
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Year: 1996 PMID： 8926336 DOI： 10.1016/0165-022x(95)00028-p
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X