| Literature DB >> 8925086 |
K Yamashita1, H Murakami, T Okuda, M Motohashi.
Abstract
A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of pioglitazone and its metabolites (M-I to M-V) in human serum and urine was developed. The method for serum involved the solid-phase and liquid-liquid extraction. Urine with and without enzymatic hydrolysis using beta-glucuronidase was treated with liquid-liquid extraction. The compounds in the extract were analyzed using HPLC with UV detection at 269 nm. The detection limits of pioglitazone, M-I, M-II, M-III, M-IV, and M-V in serum were 0.01-0.05 micrograms/ml, those in urine were 0.1-0.5 micrograms/ml, and those in urine after enzymatic hydrolysis were 0.3-0.5 micrograms/ml, respectively. The method was applied to the clinical trials of pioglitazone.Entities:
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Year: 1996 PMID： 8925086 DOI： 10.1016/0378-4347(95)00440-8
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495