Literature DB >> 8921903

Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis.

B G Gordhan1, S J Andersen, A R De Meyer, V Mizrahi.   

Abstract

Gene replacement was achieved by homologous recombination in Mycobacterium smegmatis (Ms) using a cloned segment of the polA gene (encoding the DNA polymerase I) disrupted within the region encoding the C-terminal DNA polymerase domain by a kanamycin-resistance marker. The Ms polA755:aph mutant thus generated displayed a phenotype of hypersensitivity to DNA damage induced by UV irradiation and by hydrogen peroxide challenge.

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Year:  1996        PMID： 8921903     DOI： 10.1016/0378-1119(96)00350-2

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  16 in total

1.  The stringent response of Mycobacterium tuberculosis is required for long-term survival.

Authors:  T P Primm; S J Andersen; V Mizrahi; D Avarbock; H Rubin; C E Barry
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

2.  Construction and phenotypic characterization of an auxotrophic mutant of Mycobacterium tuberculosis defective in L-arginine biosynthesis.

Authors:  Bhavna G Gordhan; Debbie A Smith; Heidi Alderton; Ruth A McAdam; Gregory J Bancroft; Valerie Mizrahi
Journal:  Infect Immun       Date:  2002-06       Impact factor: 3.441

3.  The DNA Repair Repertoire of Mycobacterium smegmatis FenA Includes the Incision of DNA 5' Flaps and the Removal of 5' Adenylylated Products of Aborted Nick Ligation.

Authors:  Maria Loressa Uson; Shreya Ghosh; Stewart Shuman
Journal:  J Bacteriol       Date:  2017-08-08       Impact factor: 3.490

4.  A genome-wide analysis of multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis Beijing genotype.

Authors:  Wei Wu; Huajun Zheng; Lu Zhang; Zilu Wen; Shulin Zhang; Hao Pei; Guohua Yu; Yongqiang Zhu; Zhenling Cui; Zhongyi Hu; Honghai Wang; Yao Li
Journal:  Mol Genet Genomics       Date:  2013-06-26       Impact factor: 3.291

5.  Characterization of the cydAB-encoded cytochrome bd oxidase from Mycobacterium smegmatis.

Authors:  B D Kana; E A Weinstein; D Avarbock; S S Dawes; H Rubin; V Mizrahi
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

6.  Expression of Mycobacterium smegmatis pyrazinamidase in Mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides.

Authors:  H I Boshoff; V Mizrahi
Journal:  J Bacteriol       Date:  2000-10       Impact factor: 3.490

7.  Functional characterization of a vitamin B12-dependent methylmalonyl pathway in Mycobacterium tuberculosis: implications for propionate metabolism during growth on fatty acids.

Authors:  Suzana Savvi; Digby F Warner; Bavesh D Kana; John D McKinney; Valerie Mizrahi; Stephanie S Dawes
Journal:  J Bacteriol       Date:  2008-03-28       Impact factor: 3.490

Review 8.  DNA Replication in Mycobacterium tuberculosis.

Authors:  Zanele Ditse; Meindert H Lamers; Digby F Warner
Journal:  Microbiol Spectr       Date:  2017-03

9.  Purification, gene cloning, targeted knockout, overexpression, and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis.

Authors:  H I Boshoff; V Mizrahi
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

10.  Characterization of three mycobacterial DinB (DNA polymerase IV) paralogs highlights DinB2 as naturally adept at ribonucleotide incorporation.

Authors:  Heather Ordonez; Maria Loressa Uson; Stewart Shuman
Journal:  Nucleic Acids Res       Date:  2014-09-08       Impact factor: 16.971
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