Literature DB >> 8919817

Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR.

M Briglia1, R I Eggen, W M de Vos, J D van Elsas.   

Abstract

A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.

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Year:  1996        PMID： 8919817      PMCID： PMC167922          DOI： 10.1128/aem.62.4.1478-1480.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Effect of ionic and nonionic detergents on the Taq polymerase.

Authors:  R S Weyant; P Edmonds; B Swaminathan
Journal:  Biotechniques       Date:  1990-09       Impact factor: 1.993

2.  Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction.

Authors:  Y L Tsai; B H Olson
Journal:  Appl Environ Microbiol       Date:  1992-02       Impact factor: 4.792

3.  Maximizing sensitivity and specificity of PCR by pre-amplification heating.

Authors:  R T D'Aquila; L J Bechtel; J A Videler; J J Eron; P Gorczyca; J C Kaplan
Journal:  Nucleic Acids Res       Date:  1991-07-11       Impact factor: 16.971

4.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.

Authors:  C Picard; C Ponsonnet; E Paget; X Nesme; P Simonet
Journal:  Appl Environ Microbiol       Date:  1992-09       Impact factor: 4.792

6.  Phylogenetic evidence for transfer of pentachlorophenol-mineralizing Rhodococcus chlorophenolicus PCP-I(T) to the genus Mycobacterium.

Authors:  M Briglia; R I Eggen; D J Van Elsas; W M De Vos
Journal:  Int J Syst Bacteriol       Date:  1994-07
  6 in total
  3 in total

1.  16S rRNA gene-based detection of tetrachloroethene-dechlorinating Desulfuromonas and Dehalococcoides species.

Authors:  F E Löffler; Q Sun; J Li; J M Tiedje
Journal:  Appl Environ Microbiol       Date:  2000-04       Impact factor: 4.792

2.  Fate of the biological control agent Pseudomonas aureofaciens TX-1 after application to turfgrass.

Authors:  W V Sigler; C H Nakatsu; Z J Reicher; R F Turco
Journal:  Appl Environ Microbiol       Date:  2001-08       Impact factor: 4.792

3.  Introduction of anaerobic dechlorinating bacteria into soil slurry microcosms and nested-PCR monitoring.

Authors:  S el Fantroussi; J Mahillon; H Naveau; S N Agathos
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792
  3 in total

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