Literature DB >> 8918907

LacZ staining in paraffin-embedded tissue sections.

P J Hendrikx1, J Vermeulen, A Hagenbeek, M Vermey, A C Martens.   

Abstract

Femora and tibiae of rats carrying leukemia from a LacZ-marked acute promyelocytic leukemia-derived leukemic cell line (LT12NL15) were decalcified using EDTA and routinely embedded in paraffin. Sections were used to develop for the first time an immunostaining method for LacZ, employing catalyzed reporter deposition (CARD) based on the deposition of biotinylated tyramine. This method was used to study homing and adhesion of leukemic cells.

Entities:  

Mesh:

Substances:

Year:  1996        PMID: 8918907     DOI: 10.1177/44.11.8918907

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  1 in total

1.  Techniques for following labeled cells in vivo: use of X/Y FISH, techniques to optimize fluorescent detection, and beta-galactosidase detection.

Authors:  Michael Craig; Michael Schumacher; Yana Zavros
Journal:  Methods Mol Biol       Date:  2012
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.