Literature DB >> 8918809

Ordered shotgun sequencing of a 135 kb Xq25 YAC containing ANT2 and four possible genes, including three confirmed by EST matches.

C N Chen1, Y Su, P Baybayan, A Siruno, R Nagaraja, R Mazzarella, D Schlessinger, E Chen.   

Abstract

Ordered shotgun sequencing (OSS) has been successfully carried out with an Xq25 YAC substrate. yWXD703 DNA was subcloned into lambda phage and sequences of insert ends of the lambda subclones were used to generate a map to select a minimum tiling path of clones to be completely sequenced. The sequence of 135 038 nt contains the entire ANT2 cDNA as well as four other candidates suggested by computer-assisted analyses. One of the putative genes is homologous to a gene implicated in Graves' disease and it, ANT2 and two others are confirmed by EST matches. The results suggest that OSS can be applied to YACs in accord with earlier simulations and further indicate that the sequence of the YAC accurately reflects the sequence of uncloned human DNA.

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Year:  1996        PMID： 8918809      PMCID： PMC146183          DOI： 10.1093/nar/24.20.4034

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

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Authors:  G B Singh; S A Krawetz
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4.  Localization of the adenine nucleotide translocase gene ANT2 to chromosome Xq24-q25 with tight linkage to DXS425.

Authors:  K Schiebel; A Mertz; M Winkelmann; R Nagaraja; G Rappold
Journal:  Genomics       Date:  1994-12       Impact factor: 5.736

5.  Alu and L1 sequence distributions in Xq24-q28 and their comparative utility in YAC contig assembly and verification.

Authors:  G Porta; I Zucchi; L Hillier; P Green; V Nowotny; M D'Urso; D Schlessinger
Journal:  Genomics       Date:  1993-05       Impact factor: 5.736

Review 6.  The isochore organization of the human genome and its evolutionary history--a review.

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8.  The construction and analysis of M13 libraries prepared from YAC DNA.

Authors:  M Vaudin; A Roopra; L Hillier; R Brinkman; J Sulston; R K Wilson; R H Waterston
Journal:  Nucleic Acids Res       Date:  1995-02-25       Impact factor: 16.971

9.  Number of CpG islands and genes in human and mouse.

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Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-15       Impact factor: 11.205

10.  Sequence and pattern of expression of a bovine homologue of a human mitochondrial transport protein associated with Grave's disease.

Authors:  G Fiermonte; M J Runswick; J E Walker; F Palmieri
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4.  Genomic sequence, structural organization, molecular evolution, and aberrant rearrangement of promyelocytic leukemia zinc finger gene.

Authors:  T Zhang; H Xiong; L X Kan; C K Zhang; X F Jiao; G Fu; Q H Zhang; L Lu; J H Tong; B W Gu; M Yu; J X Liu; J Licht; S Waxman; A Zelent; E Chen; S J Chen
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5.  Recovery and potential utility of YACs as circular YACs/BACs.

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  5 in total

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