Literature DB >> 8918196

Methods and reagents. Purification of GST fusion proteins.

P N Hengen1.   

Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds.reagnts, available on the Internet. This month's column discusses some common problems associated with the construction of protein fusions with glutathione S-transferase and with hybridization membranes. For details on how to partake in the newsgroup, see the accompanying box.

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Year:  1996        PMID： 8918196

Source DB:  PubMed          Journal:  Trends Biochem Sci        ISSN： 0968-0004            Impact factor:   13.807


  5 in total

1.  Binding of LARP6 to the conserved 5' stem-loop regulates translation of mRNAs encoding type I collagen.

Authors:  Le Cai; Dillon Fritz; Lela Stefanovic; Branko Stefanovic
Journal:  J Mol Biol       Date:  2009-11-13       Impact factor: 5.469

2.  Capping of the N-terminus of PSD-95 by calmodulin triggers its postsynaptic release.

Authors:  Yonghong Zhang; Lucas Matt; Tommaso Patriarchi; Zulfiqar A Malik; Dhrubajyoti Chowdhury; Deborah K Park; Alessandra Renieri; James B Ames; Johannes W Hell
Journal:  EMBO J       Date:  2014-04-04       Impact factor: 11.598

3.  Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain).

Authors:  Miles C Scotcher; Jeffery A McGarvey; Eric A Johnson; Larry H Stanker
Journal:  PLoS One       Date:  2009-03-17       Impact factor: 3.240

4.  A mitochondrial kinase complex is essential to mediate an ERK1/2-dependent phosphorylation of a key regulatory protein in steroid biosynthesis.

Authors:  Cecilia Poderoso; Daniela P Converso; Paula Maloberti; Alejandra Duarte; Isabel Neuman; Soledad Galli; Fabiana Cornejo Maciel; Cristina Paz; María C Carreras; Juan J Poderoso; Ernesto J Podestá
Journal:  PLoS One       Date:  2008-01-16       Impact factor: 3.240

Review 5.  Recombinant viral proteins for use in diagnostic ELISAs to detect virus infection.

Authors:  Kelly-Anne Spencer; Fernando A Osorio; Julian A Hiscox
Journal:  Vaccine       Date:  2007-03-06       Impact factor: 3.641
  5 in total

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