Embryonic bursa development in vitro.

An in vitro culture system for embryonic chicken bursa is described, in which development, as measured by follicle formation (site of B lymphocyte development), position of large basophilic cells (stem cells), and presence of lymphocytes and cells containing cytoplasmic immunoglobulin (B cells), was comparable to that of the bursa in vivo. Using this system, preliminary studies on B lymphocyte differentiation were made. These support the view that bursal lymphocytes develop from precursors that migrate through the surrounding mesenchyme and into the follicles, but indicate that at least some of these cells may be "pre-B cells" rather than pluripotent stem cells.