C A Wright1, L Taylor, K Cooper. 1. Department of Anatomical Pathology, South African Institute for Medical Research, Johannesburg.
Abstract
OBJECTIVE: To determine the human papillomavirus (HPV) subtypes in vulvovaginal warts in prepubescent children. DESIGN: Histopathology case series. SETTING: Outpatient and gynaecology clinics of hospitals in the greater Johannesburg area. PATIENTS: All cases of vulvovaginal warts diagnosed in children under the age of 12 years received at the South African Institute for Medical Research, Johannesburg, during the period 1 January 1991 to 31 December 1993. MAIN OUTCOME MEASURES: Positivity for "genital' HPV types 6, 11, 16, 18, 31, 33 and 35 using non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR). RESULTS: Eight of the 9 vulvovaginal warts contained HPV 11 when assessed by means of NISH (89%). PCR amplified HPV DNA in all 9 (100%) of the biopsies. CONCLUSION: Detection of genital subtypes of HPV in childhood condylomata acuminata points strongly to sexual abuse, but should only be used as a guide to further investigation by a multidisciplinary team.
OBJECTIVE: To determine the human papillomavirus (HPV) subtypes in vulvovaginal warts in prepubescent children. DESIGN: Histopathology case series. SETTING:Outpatient and gynaecology clinics of hospitals in the greater Johannesburg area. PATIENTS: All cases of vulvovaginal warts diagnosed in children under the age of 12 years received at the South African Institute for Medical Research, Johannesburg, during the period 1 January 1991 to 31 December 1993. MAIN OUTCOME MEASURES: Positivity for "genital' HPV types 6, 11, 16, 18, 31, 33 and 35 using non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR). RESULTS: Eight of the 9 vulvovaginal warts contained HPV 11 when assessed by means of NISH (89%). PCR amplified HPV DNA in all 9 (100%) of the biopsies. CONCLUSION: Detection of genital subtypes of HPV in childhood condylomata acuminata points strongly to sexual abuse, but should only be used as a guide to further investigation by a multidisciplinary team.
Authors: Cecily Banura; Florence M Mirembe; Jackson Orem; Anthony K Mbonye; Simon Kasasa; Edward K Mbidde Journal: Infect Agent Cancer Date: 2013-07-10 Impact factor: 2.965