Literature DB >> 8913483

Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology.

B L Robbins1, B H Waibel, A Fridland.   

Abstract

This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug. This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV. The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations. The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.

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Year:  1996        PMID： 8913483      PMCID： PMC163594     

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  22 in total

1.  A randomized controlled trial of a reduced daily dose of zidovudine in patients with the acquired immunodeficiency syndrome. The AIDS Clinical Trials Group.

Authors:  M A Fischl; C B Parker; C Pettinelli; M Wulfsohn; M S Hirsch; A C Collier; D Antoniskis; M Ho; D D Richman; E Fuchs
Journal:  N Engl J Med       Date:  1990-10-11       Impact factor: 91.245

2.  A sensitive nonisotopic method for the determination of intracellular azidothymidine 5'-mono-, 5'-di-, and 5'-triphosphate.

Authors:  T Toyoshima; S Kimura; S Muramatsu; H Takahagi; K Shimada
Journal:  Anal Biochem       Date:  1991-08-01       Impact factor: 3.365

Review 3.  AIDS Clinical Trials Group: phase I/II study of combination 2',3'-dideoxycytidine and zidovudine in patients with acquired immunodeficiency syndrome (AIDS) and advanced AIDS-related complex.

Authors:  T C Meng; M A Fischl; D D Richman
Journal:  Am J Med       Date:  1990-05-21       Impact factor: 4.965

4.  2',3'-dideoxyinosine (ddI) in patients with the acquired immunodeficiency syndrome or AIDS-related complex. A phase I trial.

Authors:  J S Lambert; M Seidlin; R C Reichman; C S Plank; M Laverty; G D Morse; C Knupp; C McLaren; C Pettinelli; F T Valentine
Journal:  N Engl J Med       Date:  1990-05-10       Impact factor: 91.245

5.  Relationship of deoxynucleotide changes to inhibition of DNA synthesis induced by the antiretroviral agent 3'-azido-3'-deoxythymidine and release of its monophosphate by human lymphoid cells (CCRF-CEM).

Authors:  A Fridland; M C Connelly; R Ashmun
Journal:  Mol Pharmacol       Date:  1990-05       Impact factor: 4.436

6.  3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro.

Authors:  H Mitsuya; K J Weinhold; P A Furman; M H St Clair; S N Lehrman; R C Gallo; D Bolognesi; D W Barry; S Broder
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

7.  A method for the quantification of intracellular zidovudine nucleotides.

Authors:  H Kuster; M Vogt; B Joos; V Nadai; R Lüthy
Journal:  J Infect Dis       Date:  1991-10       Impact factor: 5.226

8.  The efficacy of azidothymidine (AZT) in the treatment of patients with AIDS and AIDS-related complex. A double-blind, placebo-controlled trial.

Authors:  M A Fischl; D D Richman; M H Grieco; M S Gottlieb; P A Volberding; O L Laskin; J M Leedom; J E Groopman; D Mildvan; R T Schooley
Journal:  N Engl J Med       Date:  1987-07-23       Impact factor: 91.245

9.  Phase I/II study of 3TC (lamivudine) in HIV-positive, asymptomatic or mild AIDS-related complex patients: sustained reduction in viral markers. The Lamivudine European HIV Working Group.

Authors:  D Ingrand; J Weber; C A Boucher; C Loveday; C Robert; A Hill; N Cammack
Journal:  AIDS       Date:  1995-12       Impact factor: 4.177

10.  Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase.

Authors:  P A Furman; J A Fyfe; M H St Clair; K Weinhold; J L Rideout; G A Freeman; S N Lehrman; D P Bolognesi; S Broder; H Mitsuya
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205
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  12 in total

Review 1.  Direct and indirect quantification of phosphate metabolites of nucleoside analogs in biological samples.

Authors:  Nagsen Gautam; Jawaher Abdullah Alamoudi; Sushil Kumar; Yazen Alnouti
Journal:  J Pharm Biomed Anal       Date:  2019-10-03       Impact factor: 3.935

2.  High-performance liquid chromatographic determination of ribavirin in whole blood to assess disposition in erythrocytes.

Authors:  M Homma; A L Jayewardene; J Gambertoglio; F Aweeka
Journal:  Antimicrob Agents Chemother       Date:  1999-11       Impact factor: 5.191

3.  Simultaneous quantification of intracellular lamivudine and abacavir triphosphate metabolites by LC-MS/MS.

Authors:  Nagsen Gautam; Zhiyi Lin; Mary G Banoub; Nathan A Smith; Audai Maayah; JoEllyn McMillan; Howard E Gendelman; Yazen Alnouti
Journal:  J Pharm Biomed Anal       Date:  2018-02-20       Impact factor: 3.935

4.  Determination of zidovudine triphosphate intracellular concentrations in peripheral blood mononuclear cells from human immunodeficiency virus-infected individuals by tandem mass spectrometry.

Authors:  E Font; O Rosario; J Santana; H García; J P Sommadossi; J F Rodriguez
Journal:  Antimicrob Agents Chemother       Date:  1999-12       Impact factor: 5.191

5.  Simultaneous quantitation of the 5'-triphosphate metabolites of zidovudine, lamivudine, and stavudine in peripheral mononuclear blood cells of HIV infected patients by high-performance liquid chromatography tandem mass spectrometry.

Authors:  J D Moore; G Valette; A Darque; X J Zhou; J P Sommadossi
Journal:  J Am Soc Mass Spectrom       Date:  2000-12       Impact factor: 3.109

6.  Quantitation of intracellular triphosphate of emtricitabine in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients.

Authors:  A Darque; G Valette; F Rousseau; L H Wang; J P Sommadossi; X J Zhou
Journal:  Antimicrob Agents Chemother       Date:  1999-09       Impact factor: 5.191

7.  Intracellular nucleotides of (-)-2',3'-deoxy-3'-thiacytidine in peripheral blood mononuclear cells of a patient infected with human immunodeficiency virus.

Authors:  C Solas; Y F Li; M Y Xie; J P Sommadossi; X J Zhou
Journal:  Antimicrob Agents Chemother       Date:  1998-11       Impact factor: 5.191

8.  Intracellular pharmacokinetics of once versus twice daily zidovudine and lamivudine in adolescents.

Authors:  Patricia M Flynn; John Rodman; Jane C Lindsey; Brian Robbins; Edmund Capparelli; Katherine M Knapp; Jose F Rodriguez; James McNamara; Leslie Serchuck; Barbara Heckman; Jaime Martinez
Journal:  Antimicrob Agents Chemother       Date:  2007-07-30       Impact factor: 5.191

Review 9.  Intracellular phosphorylation of zidovudine (ZDV) and other nucleoside reverse transcriptase inhibitors (RTI) used for human immunodeficiency virus (HIV) infection.

Authors:  K Peter; J G Gambertoglio
Journal:  Pharm Res       Date:  1998-06       Impact factor: 4.200

10.  Determination of ddATP levels in human immunodeficiency virus-infected patients treated with dideoxyinosine.

Authors:  Cecile Le Saint; Raphael Terreux; Daniele Duval; Jacques Durant; Helene Ettesse; Pierre Dellamonica; Roger Guedj; Jean Pierre Vincent; Anny Cupo
Journal:  Antimicrob Agents Chemother       Date:  2004-02       Impact factor: 5.191
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