| Literature DB >> 8910652 |
A Ivanov1, O Vapalahti, H Lankinen, E Tkachenko, A Vaheri, B Niklasson, A Lundkvist.
Abstract
A novel enzyme-linked immunosorbent assay, BLA IgM-ELISA, based on baculovirus-expressed Puumala (PUU) virus nucleocapsid protein, was developed for rapid diagnosis of nephropathia epidemica. The recombinant antigen (bac-PUU-N) was purified to homogeneity by HPLC and conjugated to biotin. The biotin-streptavidin system, in combination with the mu-capture technique, rendered the BLA IgM-ELISA a sensitivity similar to or higher than that of PUU virus IgM mu-capture ELISA based on native antigen. The assay was shown to be highly specific when evaluated using a panel of 160 patient and control sera.Entities:
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Year: 1996 PMID： 8910652 DOI： 10.1016/0166-0934(96)02090-3
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014