M Tei1, K Nishida, S Kinoshita. 1. Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Abstract
PURPOSE: We investigated the efficacy of polymerase chain reaction (PCR) in diagnosing atypical epithelial keratitis after a penetrating keratoplasty (PKP) for herpetic corneal scarring. METHODS: We examined three eyes of three patients with keratitis after a PKP for herpetic corneal scarring. The clinical presentation of the keratitis was atypical. DNA extracted from the tears was subjected to PCR and Southern blot hybridization. RESULTS: Herpes simplex virus DNA was identified in all cases of presumptive recurrent herpetic keratitis. CONCLUSION: Polymerase chain reaction is rapid and effective for diagnosing atypical recurrent herpetic keratitis.
PURPOSE: We investigated the efficacy of polymerase chain reaction (PCR) in diagnosing atypical epithelial keratitis after a penetrating keratoplasty (PKP) for herpetic corneal scarring. METHODS: We examined three eyes of three patients with keratitis after a PKP for herpetic corneal scarring. The clinical presentation of the keratitis was atypical. DNA extracted from the tears was subjected to PCR and Southern blot hybridization. RESULTS: Herpes simplex virus DNA was identified in all cases of presumptive recurrent herpetic keratitis. CONCLUSION: Polymerase chain reaction is rapid and effective for diagnosing atypical recurrent herpetic keratitis.
Authors: N Koizumi; K Nishida; W Adachi; M Tei; Y Honma; A Dota; C Sotozono; N Yokoi; S Yamamoto; S Kinoshita Journal: Br J Ophthalmol Date: 1999-08 Impact factor: 4.638