Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera.

Literature DB >> 8905315

Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera.

D Deruaz¹, P Eid, J Deruaz, A Sempéré, C Bourgouin, F Rodhain, C Pérez-Eid.

Abstract

A modified ELISA was developed for the detection of anti-Borrelia burgdorferi (Bb) IgG antibodies in wild animal sera based on an Enzyme-Labelled-protein G Assay (ELGA). Microplates were coated with an extract of Bb sensu stricto strain (SVI) as antigen. Specific antibodies of the serum samples were detected by a peroxidase-labelled-protein G. Using comparative immunodiagnosis by means of a passive hemagglutination test (HA), ELGA was tested on 82 roe-deer blood samples. A correlation was found between the two methods (r = 0.66). Good reproducibility of titers was observed by ELGA technique. A minimal cross-reactivity was discovered with Leptospira. ELGA could facilitate the recognition of specific antibodies in collections of wild animal sera.

Entities: Species

Mesh：

Substances：

Year: 1996 PMID： 8905315 DOI： 10.1007/bf00144006

Source DB: PubMed Journal: Eur J Epidemiol ISSN： 0393-2990 Impact factor: 8.082

17 in total

1. Infection of Ixodes ricinus (Acari:Ixodidae) by Borrelia burgdorferi in Ile de France.

Authors: E Zhioua; D Postic; F Rodhain; C Perez-Eid
Journal: J Med Entomol Date: 1996-07 Impact factor: 2.278

2. A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties.

Authors: B Akerström; L Björck
Journal: J Biol Chem Date: 1986-08-05 Impact factor: 5.157

Review 3. Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.

Authors: J J Langone
Journal: Adv Immunol Date: 1982 Impact factor: 3.543

4. Streptococcal protein G, expressed by streptococci or by Escherichia coli, has separate binding sites for human albumin and IgG.

Authors: L Björck; W Kastern; G Lindahl; K Widebäck
Journal: Mol Immunol Date: 1987-10 Impact factor: 4.407

5. Antibody response in white-footed mice (Peromyscus leucopus) experimentally infected with the Lyme disease spirochete (Borrelia burgdorferi).

Authors: T G Schwan; K K Kime; M E Schrumpf; J E Coe; W J Simpson
Journal: Infect Immun Date: 1989-11 Impact factor: 3.441

6. Comparison of an indirect fluorescent-antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease.

Authors: L A Magnarelli; J M Meegan; J F Anderson; W A Chappell
Journal: J Clin Microbiol Date: 1984-08 Impact factor: 5.948

7. Borrelia infections of ruminants in Europe.

Authors: G Uilenberg; H K Hinaidy; N M Perié; T Feenstra
Journal: Vet Q Date: 1988-01 Impact factor: 3.320

8. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis.

Authors: G Baranton; D Postic; I Saint Girons; P Boerlin; J C Piffaretti; M Assous; P A Grimont
Journal: Int J Syst Bacteriol Date: 1992-07

Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera.

1. Infection of Ixodes ricinus (Acari:Ixodidae) by Borrelia burgdorferi in Ile de France.

2. A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties.

Review 3. Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.

4. Streptococcal protein G, expressed by streptococci or by Escherichia coli, has separate binding sites for human albumin and IgG.

5. Antibody response in white-footed mice (Peromyscus leucopus) experimentally infected with the Lyme disease spirochete (Borrelia burgdorferi).

6. Comparison of an indirect fluorescent-antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease.

7. Borrelia infections of ruminants in Europe.

8. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis.

9. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies.

10. Isolation and cultivation of Lyme disease spirochetes.

1. Detection of spirochaetes of Borrelia burgdorferi complexe in the skin of cervids by PCR and culture.

2. Serological signature of tick-borne pathogens in Scandinavian brown bears over two decades.