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Literature DB >> 8904451

Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas.

M F Baay¹, W G Quint, J Koudstaal, H Hollema, J M Duk, M P Burger, E Stolz, P Herbrink.

Abstract

We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA Primers
DNA, Viral

Year: 1996 PMID： 8904451 PMCID： PMC228883 DOI： 10.1128/jcm.34.3.745-747.1996

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

1. Amplification of human papillomavirus DNA sequences by using conserved primers.

Authors: L Gregoire; M Arella; J Campione-Piccardo; W D Lancaster
Journal: J Clin Microbiol Date: 1989-12 Impact factor: 5.948

2. Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction.

Authors: J Ben-Ezra; D A Johnson; J Rossi; N Cook; A Wu
Journal: J Histochem Cytochem Date: 1991-03 Impact factor: 2.479

3. Detection of loss of heterozygosity in formalin-fixed paraffin-embedded tumor specimens by the polymerase chain reaction.

Authors: A B Bianchi; N M Navone; C J Conti
Journal: Am J Pathol Date: 1991-02 Impact factor: 4.307

4. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

5. General primer-mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas.

Authors: A J van den Brule; P J Snijders; R L Gordijn; O P Bleker; C J Meijer; J M Walboomers
Journal: Int J Cancer Date: 1990-04-15 Impact factor: 7.396

6. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes.

Authors: P J Snijders; A J van den Brule; H F Schrijnemakers; G Snow; C J Meijer; J M Walboomers
Journal: J Gen Virol Date: 1990-01 Impact factor: 3.891

7. Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers.

Authors: R M Resnick; M T Cornelissen; D K Wright; G H Eichinger; H S Fox; J ter Schegget; M M Manos
Journal: J Natl Cancer Inst Date: 1990-09-19 Impact factor: 13.506

8. The causal link between human papillomavirus and invasive cervical cancer: a population-based case-control study in Colombia and Spain.

Authors: N Muñoz; F X Bosch; S de Sanjosé; L Tafur; I Izarzugaza; M Gili; P Viladiu; C Navarro; C Martos; N Ascunce
Journal: Int J Cancer Date: 1992-11-11 Impact factor: 7.396

9. Detection of the human papilloma virus type 16 mRNA-transcripts in cytological abnormal scrapings.

Authors: C Falcinelli; E Claas; B Kleter; W G Quint
Journal: J Med Virol Date: 1992-06 Impact factor: 2.327

10. Detection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers.

Authors: H Yoshikawa; T Kawana; K Kitagawa; M Mizuno; H Yoshikura; A Iwamoto
Journal: Jpn J Cancer Res Date: 1991-05

76 in total

1. A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction.

Authors: K Szuhai; E Sandhaus; S M Kolkman-Uljee; M Lemaître; J C Truffert; R W Dirks; H J Tanke; G J Fleuren; E Schuuring; A K Raap
Journal: Am J Pathol Date: 2001-11 Impact factor: 4.307

2. Evaluation of the polyclonal ELISA HPV serology assay as a biomarker for human papillomavirus exposure.

Authors: Sarah E Coseo; Carolina Porras; Lori E Dodd; Allan Hildesheim; Ana Cecilia Rodriguez; Mark Schiffman; Rolando Herrero; Sholom Wacholder; Paula Gonzalez; Mark E Sherman; Silvia Jimenez; Diane Solomon; Catherine Bougelet; Leen-Jan van Doorn; Wim Quint; Mahboobeh Safaeian
Journal: Sex Transm Dis Date: 2011-10 Impact factor: 2.830

3. High prevalence of oncogenic HPV-16 in cervical smears of asymptomatic women of eastern Uttar Pradesh, India: a population-based study.

Authors: Shikha Srivastava; Sadhana Gupta; Jagat Kumar Roy
Journal: J Biosci Date: 2012-03 Impact factor: 1.826

4. Evaluation of combined general primer-mediated PCR sequencing and type-specific PCR strategies for determination of human papillomavirus genotypes in cervical cell specimens.

Authors: Véronique Fontaine; Corinne Mascaux; Christine Weyn; Aurore Bernis; Nathalie Celio; Philippe Lefèvre; Leonard Kaufman; Christian Garbar
Journal: J Clin Microbiol Date: 2007-01-17 Impact factor: 5.948