Literature DB >> 8901464

Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes.

G Wallukat1, H U Simon, W D Müller, I Wolf.   

Abstract

A monoclonal antibody with a high affinity for digitoxin (KA = 0.50 nM) and digoxin (KA = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication.

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Year:  1996        PMID： 8901464     DOI： 10.1007/bf00240040

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  22 in total

1.  Antisera specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the Na,K-ATPase: differential expression of alpha and beta subunits in rat tissue membranes.

Authors:  A W Shyjan; R Levenson
Journal:  Biochemistry       Date:  1989-05-30       Impact factor: 3.162

Review 2.  The fundamental mechanism of inotropic action of digitalis.

Authors:  T W Smith
Journal:  Therapie       Date:  1989 Nov-Dec       Impact factor: 2.070

3.  Kinetics of the Fab fragments of digoxin antibodies and of bound digoxin in patients with severe digoxin intoxication.

Authors:  W Schaumann; B Kaufmann; P Neubert; A Smolarz
Journal:  Eur J Clin Pharmacol       Date:  1986       Impact factor: 2.953

4.  Digoxin-specific antibodies.

Authors:  V P Butler; J P Chen
Journal:  Proc Natl Acad Sci U S A       Date:  1967-01       Impact factor: 11.205

5.  Immunopharmacological activity of monoclonal antidigitoxin.

Authors:  M Urtizberea; M Latour; L Edelman; J Carcagne; J Armand; J M Scherrmann
Journal:  Dev Biol Stand       Date:  1990

Review 6.  Myocardial phenotypic changes in Na+, K+ ATPase in left ventricular hypertrophy: pharmacological consequences.

Authors:  D Charlemagne; B Swynghedauw
Journal:  Eur Heart J       Date:  1995-05       Impact factor: 29.983

7.  High-affinity monoclonal antibodies to the cardiac glycoside, digoxin.

Authors:  M M Hunter; M N Margolies; A Ju; E Haber
Journal:  J Immunol       Date:  1982-09       Impact factor: 5.422

8.  Generation of monoclonal anti-digoxin antibodies.

Authors:  I R Kehayov; S N Tyutyulkova; S Lolov; S D Kyurkchiev
Journal:  Hybridoma       Date:  1990-10

9.  Reversal of lethal digoxin toxicity in guinea pigs using monoclonal antibodies and Fab fragments.

Authors:  P Lechat; M Mudgett-Hunter; M N Margolies; E Haber; T W Smith
Journal:  J Pharmacol Exp Ther       Date:  1984-04       Impact factor: 4.030

10.  Postnatal changes in Na,K-ATPase isoform expression in rat cardiac ventricle. Conservation of biphasic ouabain affinity.

Authors:  P A Lucchesi; K J Sweadner
Journal:  J Biol Chem       Date:  1991-05-15       Impact factor: 5.157
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