Literature DB >> 889833

Differential partitioning of tRNAs between micellar and aqueous phases: a convenient gel filtration method for separation of tRNAs.

D W Armstrong, J H Fendler.   

Abstract

Partition coefficients (Kp) of phenylalanine, glutamic acid-II and tyrosine tRNAs between water and micellar cetyltrimethylammonium chloride (CTACl) have been determined to be 5600, 520 and 3100, respectively, by gel filtration on Sephadex G-100-120. Equations for obtaining Kp values from quantitative gel filtration have also been derived. The use of this method for the separation of tRNAs is demonstrated.

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Year:  1977        PMID： 889833     DOI： 10.1016/0005-2787(77)90245-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  2 in total

1.  Charge-shift electrophoretic behavior of T-2 toxin in agarose gels.

Authors:  M I Gyongyossy-Issa; E J Christie; G G Khachatourians
Journal:  Appl Environ Microbiol       Date:  1984-05       Impact factor: 4.792

2.  Partitioning of amino acids and nucleotides between water and micellar hexadecyltrimethylammonium halides. The prebiotic significance of cationic surfaces.

Authors:  D W Armstrong; R Seguin; J H Fendler
Journal:  J Mol Evol       Date:  1977-12-29       Impact factor: 2.395
  2 in total

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