Literature DB >> 8897200

The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool.

X J Yu1, P Crocquet-Valdes, L C Cullman, D H Walker.   

Abstract

DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.

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Year:  1996        PMID： 8897200      PMCID： PMC229421          DOI： 10.1128/jcm.34.11.2853-2855.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity.

Authors:  J E Dawson; D B Fishbein; T R Eng; M A Redus; N R Green
Journal:  J Infect Dis       Date:  1990-07       Impact factor: 5.226

2.  Human infection with Ehrlichia canis, a leukocytic rickettsia.

Authors:  K Maeda; N Markowitz; R C Hawley; M Ristic; D Cox; J E McDade
Journal:  N Engl J Med       Date:  1987-04-02       Impact factor: 91.245

3.  Identification of the antigenic constituents of Ehrlichia chaffeensis.

Authors:  S M Chen; J S Dumler; H M Feng; D H Walker
Journal:  Am J Trop Med Hyg       Date:  1994-01       Impact factor: 2.345

4.  Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis.

Authors:  J E Dawson; B E Anderson; D B Fishbein; J L Sanchez; C S Goldsmith; K H Wilson; C W Duntley
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

5.  Ehrlichia chaffeensis, a new species associated with human ehrlichiosis.

Authors:  B E Anderson; J E Dawson; D C Jones; K H Wilson
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

Review 6.  The interface between research and the diagnosis of an emerging tick-borne disease, human ehrlichiosis due to Ehrlichia chaffeensis.

Authors:  J E Dawson; C K Warner; S Standaert; J G Olson
Journal:  Arch Intern Med       Date:  1996-01-22

7.  Unexplained febrile illnesses after exposure to ticks. Infection with an Ehrlichia?

Authors:  D B Fishbein; L A Sawyer; C J Holland; E B Hayes; W Okoroanyanwu; D Williams; K Sikes; M Ristic; J E McDade
Journal:  JAMA       Date:  1987-06-12       Impact factor: 56.272

8.  Human ehrlichiosis in the United States, 1985 to 1990.

Authors:  D B Fishbein; J E Dawson; L E Robinson
Journal:  Ann Intern Med       Date:  1994-05-01       Impact factor: 25.391

9.  Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates.

Authors:  J E Dawson; Y Rikihisa; S A Ewing; D B Fishbein
Journal:  J Infect Dis       Date:  1991-03       Impact factor: 5.226
  9 in total
  27 in total

1.  Molecular cloning and characterization of the 120-kilodalton protein gene of Ehrlichia canis and application of the recombinant 120-kilodalton protein for serodiagnosis of canine ehrlichiosis.

Authors:  X J Yu; J W McBride; C M Diaz; D H Walker
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

2.  Anaplasma phagocytophilum APH_1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane.

Authors:  Bernice Huang; Matthew J Troese; Shaojing Ye; Jonathan T Sims; Nathan L Galloway; Dori L Borjesson; Jason A Carlyon
Journal:  Infect Immun       Date:  2010-03-08       Impact factor: 3.441

3.  Western blot analysis of sera reactive to human monocytic ehrlichiosis and human granulocytic ehrlichiosis agents.

Authors:  A Unver; S Felek; C D Paddock; N Zhi; H W Horowitz; G P Wormser; L C Cullman; Y Rikihisa
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

4.  Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis.

Authors:  Tian Luo; Xiaofeng Zhang; William L Nicholson; Bing Zhu; Jere W McBride
Journal:  Clin Vaccine Immunol       Date:  2009-12-02

5.  Mass spectrometric analysis of Ehrlichia chaffeensis tandem repeat proteins reveals evidence of phosphorylation and absence of glycosylation.

Authors:  Abdul Wakeel; Xiaofeng Zhang; Jere W McBride
Journal:  PLoS One       Date:  2010-03-04       Impact factor: 3.240

6.  An Ehrlichia chaffeensis tandem repeat protein interacts with multiple host targets involved in cell signaling, transcriptional regulation, and vesicle trafficking.

Authors:  Abdul Wakeel; Jeeba A Kuriakose; Jere W McBride
Journal:  Infect Immun       Date:  2009-03-09       Impact factor: 3.441

7.  Characterization of the major antigenic protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and its application for serodiagnosis of ehrlichiosis.

Authors:  Tamece T Knowles; A Rick Alleman; Heather L Sorenson; David C Marciano; Edward B Breitschwerdt; Shimon Harrus; Anthony F Barbet; Myriam Bélanger
Journal:  Clin Diagn Lab Immunol       Date:  2003-07

8.  Identification of 19 polymorphic major outer membrane protein genes and their immunogenic peptides in Ehrlichia ewingii for use in a serodiagnostic assay.

Authors:  Chunbin Zhang; Qingming Xiong; Takane Kikuchi; Yasuko Rikihisa
Journal:  Clin Vaccine Immunol       Date:  2007-12-19

9.  A variable-length PCR target protein of Ehrlichia chaffeensis contains major species-specific antibody epitopes in acidic serine-rich tandem repeats.

Authors:  Tian Luo; Xiaofeng Zhang; Abdul Wakeel; Vsevolod L Popov; Jere W McBride
Journal:  Infect Immun       Date:  2008-01-22       Impact factor: 3.441

10.  Major species-specific antibody epitopes of the Ehrlichia chaffeensis p120 and E. canis p140 orthologs in surface-exposed tandem repeat regions.

Authors:  Tian Luo; Xiaofeng Zhang; Jere W McBride
Journal:  Clin Vaccine Immunol       Date:  2009-05-06
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