Literature DB >> 8897189

Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli.

K Nagayama1, Z Bi, T Oguchi, Y Takarada, S Shibata, T Honda.   

Abstract

An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and specificity of the probe versus the results of localized adherence in the HEp-2 cell assay and fluorescent actin staining assay positivity were 95.7 and 100%, respectively.

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Year:  1996        PMID： 8897189      PMCID： PMC229410          DOI： 10.1128/jcm.34.11.2819-2821.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  An inducible bundle-forming pilus of enteropathogenic Escherichia coli.

Authors:  J A Girón; A S Ho; G K Schoolnik
Journal:  Science       Date:  1991-11-01       Impact factor: 47.728

2.  Attaching and effacing enteropathogenic Escherichia coli as a cause of infantile diarrhea in Bangkok.

Authors:  P Echeverria; F Orskov; I Orskov; S Knutton; F Scheutz; J E Brown; U Lexomboon
Journal:  J Infect Dis       Date:  1991-09       Impact factor: 5.226

3.  Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea.

Authors:  A Cravioto; A Tello; A Navarro; J Ruiz; H Villafán; F Uribe; C Eslava
Journal:  Lancet       Date:  1991-02-02       Impact factor: 79.321

4.  Serotype-specific prevalence of Escherichia coli strains with EPEC adherence factor genes in infants with and without diarrhea in São Paulo, Brazil.

Authors:  T A Gomes; M A Vieira; I K Wachsmuth; P A Blake; L R Trabulsi
Journal:  J Infect Dis       Date:  1989-07       Impact factor: 5.226

5.  Screening for enteropathogenic Escherichia coli in infants with diarrhea by the fluorescent-actin staining test.

Authors:  S Knutton; A D Phillips; H R Smith; R J Gross; R Shaw; P Watson; E Price
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

Review 6.  Traditional enteropathogenic Escherichia coli of infantile diarrhea.

Authors:  R M Robins-Browne
Journal:  Rev Infect Dis       Date:  1987 Jan-Feb

7.  Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test.

Authors:  M Nishibuchi; M Arita; T Honda; T Miwatani
Journal:  J Clin Microbiol       Date:  1988-04       Impact factor: 5.948

8.  A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence.

Authors:  M S Donnenberg; J A Girón; J P Nataro; J B Kaper
Journal:  Mol Microbiol       Date:  1992-11       Impact factor: 3.501

9.  Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli.

Authors:  S Knutton; T Baldwin; P H Williams; A S McNeish
Journal:  Infect Immun       Date:  1989-04       Impact factor: 3.441

10.  Detection of a heat-labile enterotoxin gene in enterotoxigenic Escherichia coli by densitometric evaluation using highly specific enzyme-linked oligonucleotide probes.

Authors:  S Tamatsukuri; K Yamamoto; S Shibata; F Leaño; T Honda; T Miwatani
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1991-12       Impact factor: 3.267
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  2 in total

Review 1.  Diarrheagenic Escherichia coli.

Authors:  J P Nataro; J B Kaper
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

2.  Molecular variation among type IV pilin (bfpA) genes from diverse enteropathogenic Escherichia coli strains.

Authors:  T E Blank; H Zhong; A L Bell; T S Whittam; M S Donnenberg
Journal:  Infect Immun       Date:  2000-12       Impact factor: 3.441
  2 in total

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