Localization and gene expression of glucose transporters in bovine mammary gland.

Glucose uptake in the mammary gland is a rate-limiting step in milk synthesis. To study glucose transporters in the bovine mammary gland, the erythrocyte-type glucose transporter (GLUT1) and the insulin-responsive glucose transporter (GLUT4) proteins were assessed by Western blotting and immunohistochemical staining, using polyclonal antibodies against the C-terminal peptide of GLUT1 and GLUT4. Our results demonstrated that the bovine mammary gland expressed a relatively high level of GLUT1 protein, whereas GLUT4 protein was not detected in the mammary gland of either lactating or dry cows. The absence of GLUT4 may indicate that glucose transport is not regulated by insulin in the lactating and dry bovine mammary gland. The anti-GLUT1 antibody strongly stained the single layer of epithelial cells of mammary alveoli. The expression of GLUT1 mRNA was similar in the mammary gland of late lactation and non-lactating cows. However, a smaller molecular weight species (38 kDa) of GLUT1 protein was detected in the mammary gland of non-lactating cows where its abundance in crude membrane preparation was 80% higher than in lactating animals. There were no significant differences in GLUT1 mRNA in bovine mammary gland at 118 d and 181 d postpartum, however, GLUT1 protein expression tended to be greater at 118 d postpartum.