Literature DB >> 889574

Purification and properties of human kidney-cortex hexosaminidases A and B.

J E Wiktorowicz, Y C Awasthi, A Kurosky, S K Srivastava.   

Abstract

Hexosaminidases (EC 3.2.1.30) A and B from human kidney cortex were purified to homogeneity by using concanavalin A affinity chromatography, ion-exchange chromatography and gel filtration. The yield of homogeneous isoenzymes improved approx. 20-fold, giving preparations of hexosaminidases A and B with specific activities of about 200 and 325 units/mg of protein respectively. The kinetic and structural properties of kidney hexosaminidase isoenzymes were studied and compared with the hexosaminidase isoenzymes from human placenta. The amino acid composition of hexosaminidase A was significantly different from that of hexosaminidase B. In the event of success in developing enzyme-replacement therapy for Tay-Sachs and Sandhoff's diseases, this modified procedure can furnish larger amounts of homogeneous isoenzymes.

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Year:  1977        PMID： 889574      PMCID： PMC1164867          DOI： 10.1042/bj1650049

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  27 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  The subunits of human hexosaminidase A.

Authors:  E Beutler; A Yoshida; W Kuhl; J E Lee
Journal:  Biochem J       Date:  1976-12-01       Impact factor: 3.857

3.  Isolation and relationship of human hexosaminidases.

Authors:  J F Tallman; R O Brady; J M Quirk; M Villalba; A E Gal
Journal:  J Biol Chem       Date:  1974-06-10       Impact factor: 5.157

4.  Hexosaminidase-A and hexosaminidase-B: studies in Tay-Sachs' and Sandhoff's disease.

Authors:  S K Srivastava; E Beutler
Journal:  Nature       Date:  1973-02-16       Impact factor: 49.962

5.  Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.

Authors:  G E Davies; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1970-07       Impact factor: 11.205

6.  Deficient hexozaminidase activity in an exceptional case of Tay-Sachs disease with additional storage of kidney globoside in visceral organs.

Authors:  K Sandhoff; U Andreae; H Jatzkewitz
Journal:  Life Sci       Date:  1968-03-15       Impact factor: 5.037

7.  Studies on N-acetyl-beta-D-glucosaminidase of Aspergillus oryzae. I. Purification and characterization of N-acetyl-beta-D-glucosaminidase obtained from Takadiastase.

Authors:  T Mega; T Ikenaka; Y Matsushima
Journal:  J Biochem       Date:  1970-07       Impact factor: 3.387

8.  The cellular distribution of some rat-kidney glycosidases.

Authors:  R G Price; N Dance
Journal:  Biochem J       Date:  1967-11       Impact factor: 3.857

9.  Introduction of purified hexosaminidase A into Tay-Sachs leukocytes by means of immunoglobulin-coated liposomes.

Authors:  C M Cohen; G Weissmann; S Hoffstein; Y C Awasthi; S K Srivastava
Journal:  Biochemistry       Date:  1976-01-27       Impact factor: 3.162

10.  Interrelationship of hexosaminidases A and B: conformation of the common and the unique subunit theory.

Authors:  S K Srivastava; J E Wiktorowicz; Y C Awasthi
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205
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  6 in total

1.  N-acetyl-beta-d-glucosaminidase in marmoset kidney, serum and urine.

Authors:  R J Pierce; R G Price; J S Fowler
Journal:  Biochem J       Date:  1978-12-01       Impact factor: 3.857

Review 2.  Binding interactions of glycoproteins with lectins.

Authors:  J T Dulaney
Journal:  Mol Cell Biochem       Date:  1978-10-13       Impact factor: 3.396

Review 3.  The biochemical genetics of the hexosaminidase system in man.

Authors:  E Beutler
Journal:  Am J Hum Genet       Date:  1979-03       Impact factor: 11.025

4.  Enzyme replacement treatment for Tay-Sachs disease brain cells in culture utilizing concanavalin A-mediated hexosaminidase A uptake: biochemical and morphological evidence of GM2 mobilization.

Authors:  S E Brooks; L M Hoffman; M Adachi; D Amsterdam; L Schneck
Journal:  Acta Neuropathol       Date:  1980       Impact factor: 17.088

5.  Isolation of beta-N-acetylhexosaminidase from rabbit semen and its role in fertilization.

Authors:  A A Farooqui; P N Srivastava
Journal:  Biochem J       Date:  1980-12-01       Impact factor: 3.857

6.  Demonstration of cross-reacting material in Tay-Sachs disease.

Authors:  S K Srivastava; N H Ansari; L A Hawkins; J E Wiktorowicz
Journal:  Biochem J       Date:  1979-06-01       Impact factor: 3.857
  6 in total

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