OBJECTIVE: To determine whether the hypo-osmotic swelling test can assess viability of frozen spermatozoa. DESIGN: Fresh semen specimens were divided into two aliquots: one was not processed and the other was processed by swim-up. Both aliquots were then cryopreserved by the liquid nitrogen vapor method. SETTING: Andrology laboratory at a tertiary care facility. PATIENT(S): Eleven volunteers with proven fertility. INTERVENTION(S): The hypo-osmotic swelling test and the sperm viability assessment by Hoechst 33258 (Sigma Chemical Co., St. Louis, MO) and eosin-nigrosin strains were performed in each aliquot. MAIN OUTCOME MEASURE(S): The results of hypo-osmotic swelling test as indicated by swelling of sperm tails were compared with the viability results obtained by Hoechst 33258 stain before freezing and by Hoechst-33258 and eosin-nigrosin stains after freezing. RESULT(S): The hypo-osmotic swelling test and Hoechst 33258 viability results were correlated highly in unprocessed and processed fresh specimens (r = 0.95). After cryopreservation, there was no correlation between hypo-osmotic swelling test and Hoechst 33258 or between hypo-osmotic swelling test and eosin-nigrosin. Prefreeze sperm processing did not influence the post-thaw results. Viability assessed by Hoechst 33258 and eosin-nigrosin was well correlated (r = 0.72). CONCLUSION(S): The hypo-osmotic swelling test can accurately evaluate viability in fresh human spermatozoa but not in cryopreserved ones. Thus, this test cannot be used to select viable cells in cryopreserved samples for assisted reproductive procedures, such as intracytoplasmic sperm injection.
OBJECTIVE: To determine whether the hypo-osmotic swelling test can assess viability of frozen spermatozoa. DESIGN: Fresh semen specimens were divided into two aliquots: one was not processed and the other was processed by swim-up. Both aliquots were then cryopreserved by the liquid nitrogen vapor method. SETTING: Andrology laboratory at a tertiary care facility. PATIENT(S): Eleven volunteers with proven fertility. INTERVENTION(S): The hypo-osmotic swelling test and the sperm viability assessment by Hoechst 33258 (Sigma Chemical Co., St. Louis, MO) and eosin-nigrosin strains were performed in each aliquot. MAIN OUTCOME MEASURE(S): The results of hypo-osmotic swelling test as indicated by swelling of sperm tails were compared with the viability results obtained by Hoechst 33258 stain before freezing and by Hoechst-33258 and eosin-nigrosin stains after freezing. RESULT(S): The hypo-osmotic swelling test and Hoechst 33258 viability results were correlated highly in unprocessed and processed fresh specimens (r = 0.95). After cryopreservation, there was no correlation between hypo-osmotic swelling test and Hoechst 33258 or between hypo-osmotic swelling test and eosin-nigrosin. Prefreeze sperm processing did not influence the post-thaw results. Viability assessed by Hoechst 33258 and eosin-nigrosin was well correlated (r = 0.72). CONCLUSION(S): The hypo-osmotic swelling test can accurately evaluate viability in fresh human spermatozoa but not in cryopreserved ones. Thus, this test cannot be used to select viable cells in cryopreserved samples for assisted reproductive procedures, such as intracytoplasmic sperm injection.
Authors: C Briton-Jones; Q S Yeung; G C Tjer; T T Chiu; L P Cheung; S F Yim; I H Lok; C Haines Journal: J Assist Reprod Genet Date: 2001-03 Impact factor: 3.412
Authors: Ashok Agarwal; Rakesh K Sharma; Sajal Gupta; Florence Boitrelle; Renata Finelli; Neel Parekh; Damayanthi Durairajanayagam; Ramadan Saleh; Mohamed Arafa; Chak Lam Cho; Ala'a Farkouh; Amarnath Rambhatla; Ralf Henkel; Paraskevi Vogiatzi; Nicholas Tadros; Parviz Kavoussi; Edmund Ko; Kristian Leisegang; Hussein Kandil; Ayad Palani; Gianmaria Salvio; Taymour Mostafa; Osvaldo Rajmil; Saleem Ali Banihani; Samantha Schon; Tan V Le; Ponco Birowo; Gökhan Çeker; Juan Alvarez; Juan Manuel Corral Molina; Christopher C K Ho; Aldo E Calogero; Kareim Khalafalla; Mesut Berkan Duran; Shinnosuke Kuroda; Giovanni M Colpi; Armand Zini; Christina Anagnostopoulou; Edoardo Pescatori; Eric Chung; Ettore Caroppo; Fotios Dimitriadis; Germar-Michael Pinggera; Gian Maria Busetto; Giancarlo Balercia; Haitham Elbardisi; Hisanori Taniguchi; Hyun Jun Park; Israel Maldonado Rosas; Jean de la Rosette; Jonathan Ramsay; Kasonde Bowa; Mara Simopoulou; Marcelo Gabriel Rodriguez; Marjan Sabbaghian; Marlon Martinez; Mohamed Ali Sadighi Gilani; Mohamed S Al-Marhoon; Raghavender Kosgi; Rossella Cannarella; Sava Micic; Shinichiro Fukuhara; Sijo Parekattil; Sunil Jindal; Taha Abo-Almagd Abdel-Meguid; Yoshiharu Morimoto; Rupin Shah Journal: World J Mens Health Date: 2021-10-13 Impact factor: 5.400