Fumonisin B1 production by Fusarium proliferatum strain M5991 in a modified Myro liquid medium.

Fusarium proliferatum strain M5991 cultures were grown in shake flasks containing modified Myro (MM) medium (MgSO4 reduced to 0.5 g/L) plus 0, 0.25, 0.50, 0.75, 1.00, or 1.25% (v/v) hot-water and corn-hull-extract (CHE) for 69 days. After 4 days of incubation, shake flask liquid cultures with 0.75, 1.00, and 1.25% (v/v) CHE showed a reduction in pH from 6.0 to 2.6 and consumed sucrose at > 6.3 g/L/d. After 69 days of incubation, the same shake flask cultures produced over 7.8 g/L cell mass and over 990 mg/L fumonisin B1 (FB1). A minimum CHE level of 0.75% was recommended for enhanced FB1 production by F. proliferatum strain M5991. During three serial (10, 12, and 12 L) batch fermentations in MM medium + 1.00% (v/v) CHE (first batch only), F. proliferatum strain M5991 produced FB1 concentrations of 619, 659, and 375 mg/L after 35, 47, and 52 days of incubation, respectively. By analysis, a total yield of 20 g FB1 was obtained from three serial batch fermentations.