Literature DB >> 8887366

Simple procedure for estimating the efficiency of PCR.

A S Bourinbaiar1, K Ajuang-Simbiri.   

Abstract

The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.

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Year:  1996        PMID： 8887366     DOI： 10.1007/BF02762328

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  5 in total

1.  Quantification of polymerase chain reaction products by affinity-based hybrid collection.

Authors:  A C Syvänen; M Bengtström; J Tenhunen; H Söderlund
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

2.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

3.  Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

Authors:  K B Mullis; F A Faloona
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Relevance of the quantitative detection of HIV proviral sequences in PBMC of infected individuals.

Authors:  S Escaich; J Ritter; P Rougier; D Lepot; J P Lamelin; M Sepetjan; C Trepo
Journal:  AIDS Res Hum Retroviruses       Date:  1992-10       Impact factor: 2.205

5.  Biological and biochemical characterization of a cloned Leu-3- cell surviving infection with the acquired immune deficiency syndrome retrovirus.

Authors:  T M Folks; D Powell; M Lightfoote; S Koenig; A S Fauci; S Benn; A Rabson; D Daugherty; H E Gendelman; M D Hoggan
Journal:  J Exp Med       Date:  1986-07-01       Impact factor: 14.307
  5 in total
  2 in total

1.  Jurkat-Derived (J-Lat, J1.1, and Jurkat E4) and CEM-Derived T Cell Lines (8E5 and ACH-2) as Models of Reversible Proviral Latency.

Authors:  Anthony Rodari; Guido Poli; Carine Van Lint
Journal:  Methods Mol Biol       Date:  2022

2.  Serodeconversion of HIV antibody-positive AIDS patients following treatment with V-1 Immunitor.

Authors:  Orapun Metadilogkul; Vichai Jirathitikal; Aldar S Bourinbaiar
Journal:  J Biomed Biotechnol       Date:  2008-10-28
  2 in total

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