Literature DB >> 8880297

Characterization of Lactobacillus sake strains associating with production of ropy slime by randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) patterns.

J Björkroth1, J Ridell, H Korkeala.   

Abstract

Randomly amplified polymorphic DNA (RAPD) patterns and pulsed-field gel electrophoresis (PFGE) patterns were used for the characterization of ropy slime-producing Lactobacillus sake strains. The two most revealing commercially available primers (OPJ 12 and OPJ 16, Operon Inc. Alameda, USA) and two rare-cutting enzymes (AvrII and SmaI) were chosen from a pretested lot for the typing of 69 ropy slime-producing strains, 7 non-ropy isolates and 4 non-ropy reference strains. Both RAPD and PFGE patterns confirmed the group division established in previous studies and provided new information concerning ropy slime-producing strains. PFGE patterns were found to have the greatest discriminatory power, revealing the genetic variation of the main group of ropy slime-producing L. sake strains and distinguishing all non-ropy strains from slime-producers.

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Year:  1996        PMID： 8880297     DOI： 10.1016/0168-1605(96)00964-6

Source DB:  PubMed          Journal:  Int J Food Microbiol        ISSN： 0168-1605            Impact factor:   5.277


  12 in total

1.  Use of rRNA gene restriction patterns to evaluate lactic acid bacterium contamination of vacuum-packaged sliced cooked whole-meat product in a meat processing plant.

Authors:  K J Björkroth; H J Korkeala
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

2.  Genomic analysis of Clostridium botulinum group II by pulsed-field gel electrophoresis.

Authors:  S Hielm; J Björkroth; E Hyytiä; H Korkeala
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

3.  Genomic diversity within the genus Pediococcus as revealed by randomly amplified polymorphic DNA PCR and pulsed-field gel electrophoresis.

Authors:  P J Simpson; C Stanton; G F Fitzgerald; R P Ross
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

4.  Intraspecific genotypic characterization of Lactobacillus rhamnosus strains intended for probiotic use and isolates of human origin.

Authors:  M Vancanneyt; G Huys; K Lefebvre; V Vankerckhoven; H Goossens; J Swings
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792

5.  Molecular epidemiology of an outbreak of febrile gastroenteritis caused by Listeria monocytogenes in cold-smoked rainbow trout.

Authors:  M K Miettinen; A Siitonen; P Heiskanen; H Haajanen; K J Björkroth; H J Korkeala
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

6.  Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains.

Authors:  S Tynkkynen; R Satokari; M Saarela; T Mattila-Sandholm; M Saxelin
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

7.  Intraspecies genomic groups in Enterococcus faecium and their correlation with origin and pathogenicity.

Authors:  Marc Vancanneyt; Angiolella Lombardi; Christian Andrighetto; Edo Knijff; Sandra Torriani; K Johanna Björkroth; Charles M A P Franz; María R Foulquié Moreno; Hilde Revets; Luc De Vuyst; Jean Swings; Karel Kersters; Franco Dellaglio; Wilhelm H Holzapfel
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

8.  Identification and characterization of Leuconostoc carnosum, associated with production and spoilage of vacuum-packaged, sliced, cooked ham.

Authors:  K J Björkroth; P Vandamme; H J Korkeala
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

9.  Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.

Authors:  A Tilsala-Timisjärvi; T Alatossava
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

10.  Sources of Listeria monocytogenes contamination in a cold-smoked rainbow trout processing plant detected by pulsed-field gel electrophoresis typing.

Authors:  T Autio; S Hielm; M Miettinen; A M Sjöberg; K Aarnisalo; J Björkroth; T Mattila-Sandholm; H Korkeala
Journal:  Appl Environ Microbiol       Date:  1999-01       Impact factor: 4.792
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