| Literature DB >> 8879104 |
T Sugiyama1, K Suzue, M Okamoto, J Inselburg, K Tai, T Horii.
Abstract
We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.Entities:
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Year: 1996 PMID： 8879104 DOI： 10.1016/0264-410x(95)00238-v
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641