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Literature DB >> 8877129

Mapping of the H7-serospecific domain of Escherichia coli flagellin.

Abstract

The amino acid sequences responsible for H7 and H23 flagellum serology have been identified by using a genetic approach. The H7-specific domain was located between amino acids 352 and 374 of the H7 flagellin. The sequencing data also demonstrated that the difference between the H7 and H23 flagellins in this region results from a single substitution at amino acid 366 (Ser-->Thr). The common epitopes for H7 and H23 were located between amino acids 284 and 366.

Entities: Chemical Species

Mesh：

Substances：
Epitopes
Flagellin

Year: 1996 PMID： 8877129 PMCID： PMC170400 DOI： 10.1128/cdli.3.5.523-526.1996

Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X

17 in total

1. Monoclonal antibodies against serotype specific and conserved epitopes in morphotype E Escherichia coli flagellins.

Authors: G Schoenhals; C Whitfield
Journal: FEMS Microbiol Lett Date: 1990-10 Impact factor: 2.742

2. Epitope mapping of the d flagellar antigen of Salmonella muenchen.

Authors: T M Joys; F Schödel
Journal: Infect Immun Date: 1991-09 Impact factor: 3.441

3. Isolation of Escherichia coli serotype O157:H7 and other Shiga-like-toxin-producing E. coli from dairy cattle.

Authors: J G Wells; L D Shipman; K D Greene; E G Sowers; J H Green; D N Cameron; F P Downes; M L Martin; P M Griffin; S M Ostroff
Journal: J Clin Microbiol Date: 1991-05 Impact factor: 5.948

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Sequence invariance of the antigen-coding central region of the phase 1 flagellar filament gene (fliC) among strains of Salmonella typhimurium.

Authors: N H Smith; R K Selander
Journal: J Bacteriol Date: 1990-02 Impact factor: 3.490

6. Production and identification of recombinant proteins of Salmonella typhimurium and their use in detection of antibodies in experimentally challenged animals.

Authors: J Kwang; E T Littledike
Journal: FEMS Microbiol Lett Date: 1995-07-15 Impact factor: 2.742

7. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors: W N Burnette
Journal: Anal Biochem Date: 1981-04 Impact factor: 3.365

2. CD4+-T-cell responses generated during murine Salmonella enterica serovar Typhimurium infection are directed towards multiple epitopes within the natural antigen FliC.

Authors: Molly A Bergman; Lisa A Cummings; Robert C Alaniz; Laura Mayeda; Ivana Fellnerova; Brad T Cookson
Journal: Infect Immun Date: 2005-11 Impact factor: 3.441

2 in total

Mapping of the H7-serospecific domain of Escherichia coli flagellin.

1. Monoclonal antibodies against serotype specific and conserved epitopes in morphotype E Escherichia coli flagellins.

2. Epitope mapping of the d flagellar antigen of Salmonella muenchen.

3. Isolation of Escherichia coli serotype O157:H7 and other Shiga-like-toxin-producing E. coli from dairy cattle.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Sequence invariance of the antigen-coding central region of the phase 1 flagellar filament gene (fliC) among strains of Salmonella typhimurium.

6. Production and identification of recombinant proteins of Salmonella typhimurium and their use in detection of antibodies in experimentally challenged animals.

7. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

8. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

9. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Review 10. Illnesses associated with Escherichia coli O157:H7 infections. A broad clinical spectrum.

1. Mapping of Escherichia coli H27-specific epitope from H-specific polypeptides.

2. CD4+-T-cell responses generated during murine Salmonella enterica serovar Typhimurium infection are directed towards multiple epitopes within the natural antigen FliC.