| Literature DB >> 8873887 |
C S Unterkircher1, S C Yazaki, M T Shimizu, A O Jorge, Z P Camargo.
Abstract
Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.Entities:
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Year: 1996 PMID： 8873887 DOI： 10.1080/02681219680000461
Source DB: PubMed Journal: J Med Vet Mycol ISSN： 0268-1218