Localization of heme oxygenase in rat retina: effect of light adaptation.

Heme oxygenase-2 isozyme is the predominant form of heme oxygenase in rat brain by western blot analysis. Heme oxygenase-1 isozyme is not induced by light adaptation in rat retina by western blot analysis. Immunocytochemistry localizes heme oxygenase-2 in three areas of the retina: the retinal pigment epithelium, inner segment and external nuclear layers of the rat retina. Ganglion cells and cell bodies of the internal nuclear layer of the retina and Müller cells were largely unstained for heme oxygenase-2. The localization of heme oxygenase-2 in the retina implies that its function is not associated with phototransduction. Also, light adaptation does not appear to induce heme oxygenase-1, a measure of oxidative injury.