Literature DB >> 8863994

A rapid assay for determination of antiviral activity against human cytomegalovirus.

P J Hippenmeyer1, V M Dilworth.   

Abstract

RC256, the recombinant human cytomegalovirus (HCMV) which expresses beta-galactosidase, was used as a tool for rapid screening of compounds for antiviral activity. The effective concentration of antiviral compound needed to inhibit RC256 was identical to the concentrations needed to inhibit other strains of HCMV as measured by plaque reduction assay or virus yield reduction assay. Measurement of beta-galactosidase activity in infected cell lysates allowed determination of effective concentrations 48 h postinfection with results comparable to the longer, more laborious assays.

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Year:  1996        PMID： 8863994     DOI： 10.1016/0166-3542(95)00976-0

Source DB:  PubMed          Journal:  Antiviral Res        ISSN： 0166-3542            Impact factor:   5.970


  5 in total

1.  Inhibiting a dynamic viral protease by targeting a non-catalytic cysteine.

Authors:  Kaitlin R Hulce; Priyadarshini Jaishankar; Gregory M Lee; Markus-Frederik Bohn; Emily J Connelly; Kristin Wucherer; Chayanid Ongpipattanakul; Regan F Volk; Shih-Wei Chuo; Michelle R Arkin; Adam R Renslo; Charles S Craik
Journal:  Cell Chem Biol       Date:  2022-03-31       Impact factor: 9.039

2.  Discovery of Ketone-Based Covalent Inhibitors of Coronavirus 3CL Proteases for the Potential Therapeutic Treatment of COVID-19.

Authors:  Robert L Hoffman; Robert S Kania; Mary A Brothers; Jay F Davies; Rose A Ferre; Ketan S Gajiwala; Mingying He; Robert J Hogan; Kirk Kozminski; Lilian Y Li; Jonathan W Lockner; Jihong Lou; Michelle T Marra; Lennert J Mitchell; Brion W Murray; James A Nieman; Stephen Noell; Simon P Planken; Thomas Rowe; Kevin Ryan; George J Smith; James E Solowiej; Claire M Steppan; Barbara Taggart
Journal:  J Med Chem       Date:  2020-10-15       Impact factor: 7.446

3.  Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors.

Authors:  Ran He; Gordon Sandford; Gary S Hayward; William H Burns; Gary H Posner; Michael Forman; Ravit Arav-Boger
Journal:  Virol J       Date:  2011-01-26       Impact factor: 4.099

4.  A Luciferase Gene Driven by an Alphaherpesviral Promoter Also Responds to Immediate Early Antigens of the Betaherpesvirus HCMV, Allowing Comparative Analyses of Different Human Herpesviruses in One Reporter Cell Line.

Authors:  Anna Katharina Maier; Raimund Jung; Clarissa Villinger; Axel Schubert; Paul Walther; Christian Sinzger; Diana Lieber
Journal:  PLoS One       Date:  2017-01-06       Impact factor: 3.240

5.  Human cytomegalovirus-inhibitory flavonoids: studies on antiviral activity and mechanism of action.

Authors:  David L Evers; Chih-Fang Chao; Xin Wang; Zhigang Zhang; Shu-Mei Huong; Eng-Shang Huang
Journal:  Antiviral Res       Date:  2005-09-15       Impact factor: 5.970
  5 in total

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