| Literature DB >> 8861912 |
A Rowles1, J P Chong, L Brown, M Howell, G I Evan, J J Blow.
Abstract
The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.Entities:
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Year: 1996 PMID： 8861912 DOI： 10.1016/s0092-8674(00)81346-x
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582